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利用高通量筛选技术,在实验室对2种病程相关蛋白质、几丁质酶和β-1、3-葡聚糖酶以及双相过氧化氢的生成进行测试,对甜菜中11种非典型短芽孢杆菌菌株进行评估。通过温室测试,研究抑制甜菜褐斑病系统获得抗性的产生,并将这些菌株与已知的生物防治剂,如芽孢杆菌菌株Bac J和化学抗性诱导物植物抗病基因诱导剂(ASM)做比较。在实验室筛选鉴别出的短芽孢杆菌菌株203-6和203-7,可减轻70%甜菜褐斑病症状,对甜菜褐斑病症状的抑制程度与芽孢杆菌菌株Bac J和ASM类似。系统抗性的产生以2种病程相关蛋白质(PR蛋白)、几丁质酶和β-1、3-葡聚糖酶的增多为标志,并在之前产生双相过氧化氢,该现象也存在于诱发系统抗性的不相容植物病原相互作用过程中。乙二醇几丁质与苯胺蓝培养皿检测的方法,能正确鉴别出所有系统抗性的植株原位诱导因子,这些因子通过对系统获得抗性的人工感染检验可抑制甜菜褐斑病,减少70%人工感染检验的工作量。
The use of high-throughput screening technology in the laboratory of two disease-related proteins, chitinase and β-1,3-glucanase and biphasic hydrogen peroxide generation was tested on beet 11 atypical Bacillus strain was evaluated. Through the greenhouse test, the generation of resistance to sugarbeet brown spot disease system was studied, and these strains were combined with known biological control agents such as Bac J strain and plant resistance gene inducer (ASM) compare with. Screening identified strains of Bacillus brevis 203-6 and 203-7 in laboratory reduced the symptoms of brown spot disease by 70% and inhibited the symptoms of brown spot disease similar to Bacillus strains Bac J and ASM. The generation of systemic resistance is marked by an increase in two disease-related proteins (PR proteins), chitinase and beta-1, 3-glucanase and biphasic hydrogen peroxide previously produced In the process of inducing systemic resistance to incompatible plant pathogenic interactions. Ethylene glycol chitin and aniline blue petri dish detection method can correctly identify all systemic resistance in situ inducible factors, these factors through the system-acquired artificial infection test can inhibit the brown sugar beetle disease, reduce 70% Manual Infection Test workload.