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在大鼠骨髓腰4,5节段腹角内注射[~3H]亮氨酸,用液闪法测定每5mm神经节段的放射活性以确定标记蛋白在腹根、坐骨、肼总及胫神经轴突中转运的距离。脊髓内注射1M氨基嘧啶4μl对正常神经中标记蛋白的轴浆转运没有明显影响。在离脊髓70 mm处夹伤坐骨神经,可阻断标记蛋白的顺向转运,在夹伤点近心端的神经节段中形成放射活性增高的积累波。损伤后2 d,这种由标记蛋白形成的积累波开始越过损伤点向远端移动,说明损伤处已有神经再生并已有转运功能。这一结果得到了电镜形态学的验证。夹伤后5 d,标记蛋白越
The [~ 3H] leucine was injected into the ventral horn of the 4,5th segment of the rat bone marrow and the radioactivity was measured every 5 mm nerve segment by liquid scintillation to determine the presence of the marker protein in the ventral root, ischial bone, hydrazine total and tibial nerve Axis transit distance. Intradermal injection of 4 μM of 1M aminopyrimidine had no significant effect on the axonal transport of the labeled proteins in normal nerves. Sciatic nerve injury at 70 mm from the spinal cord blocked the cis-transport of the marker protein and formed an accumulation wave with increased radioactivity in the nerve segment proximal to the clamping point. At 2 days after injury, the accumulated wave formed by the marker protein began to move distally beyond the point of injury, indicating that the lesion had been regenerated and had transport function. This result was verified by electron microscopy. 5 days after injury, the more protein mark