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目的探讨番茄红素对溴代苯诱导小鼠脂质过氧化损伤的拮抗作用。方法将50只健康成年SPF级昆明雄性小鼠随机分为5组,即对照组、溴代苯模型组和低、中、高剂量番茄红素+溴代苯组,每组10只。低、中、高剂量番茄红素+溴代苯组小鼠经口灌胃浓度分别为0.33、0.67和2.00 g/L的番茄红素溶液,对照组和溴代苯模型组灌胃等体积的溶剂(含98%水、0.8%甘油、0.7%无水乙醇、0.5%玉米油),染毒容量为0.02 ml/g,每天1次,连续染毒30 d;番茄红素暴露结束0.5 h后,测定红细胞超氧化物歧化酶(SOD)活力、全血谷胱甘肽过氧化物酶(GSH-Px)活力及总抗氧化能力(T-AOC)。番茄红素暴露结束3 h后,除对照(橄榄油)组外,其余4组立即一次性灌胃染毒0.4 mg/kg的溴代苯溶液,染毒容量为0.01 ml/g,22 h后,测定肝脏中丙二醛(MDA)含量和SOD、GSH-Px活力及T-AOC。结果番茄红素暴露后,与溴代苯模型组相比,中、高剂量番茄红素+溴代苯组小鼠红细胞SOD活力、全血GSH-Px活力及全血T-AOC显著升高(P<0.05,P<0.01);溴代苯染毒后,与溴代苯模型组相比,中、高剂量番茄红素+溴代苯组小鼠肝脏中SOD活力、GSH-Px活力及T-AOC明显增强(P<0.05,P<0.01),MDA含量显著下降(P<0.05,P<0.01)。随着番茄红素暴露剂量的升高,小鼠肝脏中MDA含量呈下降趋势,而血液及肝脏中SOD、GSH-Px活力及T-AOC呈上升趋势。结论番茄红素对溴代苯引起的小鼠脂质过氧化损伤具有一定的拮抗作用。
Objective To investigate the antagonistic effect of lycopene on bromobenzene induced lipid peroxidation in mice. Methods Fifty male Kunming SPF Kunming mice were randomly divided into five groups: control group, bromobenzene model group and low, medium and high dose lycopene + bromobenzene group, with 10 in each group. Low, medium and high doses of lycopene + bromobenzene group mice oral gavage lycopene solution concentrations of 0.33,0.67 and 2.00 g / L, the control group and bromobenzene model group intragastric administration of equal volume Solvent (containing 98% water, 0.8% glycerol, 0.7% absolute ethanol and 0.5% corn oil) was used in the experiment. The exposure capacity was 0.02 ml / g once daily for 30 days. After lycopene exposure for 0.5 h The activities of erythrocyte superoxide dismutase (SOD), whole blood glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC) were measured. Three hours after the end of lycopene exposure, except for the control (olive oil) group, the other four groups were given a single dose of 0.4 mg / kg of bromobenzene solution immediately after exposure for a dose of 0.01 ml / g for 22 h The malondialdehyde (MDA) content, SOD, GSH-Px activity and T-AOC in the liver were measured. Results After lycopene exposure, the activities of SOD, GSH-Px in whole blood and T-AOC in whole blood of middle and high doses of lycopene plus bromobenzene were significantly higher than those of brominated benzene P <0.05, P <0.01). Compared with the brominated benzene model group, the activity of SOD, the activity of GSH-Px and the activity of T -AOC significantly increased (P <0.05, P <0.01), MDA content decreased significantly (P <0.05, P <0.01). With the increase of the dose of lycopene, the content of MDA in the liver of mice decreased, while the activities of SOD, GSH-Px and T-AOC in blood and liver increased. Conclusion Lycopene may antagonize lipid peroxidation induced by bromobenzene in mice.