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目的:建立以RP-HPLC色谱法测定补骨脂酊中补骨脂素含量的方法。方法:色谱柱为Spheri-5RP-18(4.6mm×250mm,5μm)柱,流动相为甲醇-水(47∶53),流速为1.0ml/min,检测波长为245nm。结果:补骨脂素检测浓度在21.20～106.00μg/ml范围内与峰面积积分值呈良好线性关系(r=0.9999),平均加样回收率为99.86%,RSD为1.06%(n=9)。结论:本方法简便快捷、灵敏度高、重现性好,可作为该制剂质量控制的方法。
OBJECTIVE: To establish a method for determination of psoralen content in Bugu Zhizhi by RP-HPLC chromatography. Method: The chromatographic column was a Spheri-5RP-18 (4.6mm×250mm, 5μm) column, the mobile phase was methanol-water (47∶53), the flow rate was 1.0ml/min, and the detection wavelength was 245nm. Results: There was a good linear relationship between the concentration of peak area and the concentration of psoralen in the range of 21.20～106.00μg/ml (r=0.9999). The average recovery rate was 99.86% and the RSD was 1.06% (n=9). . Conclusion: The method is simple, rapid, sensitive and reproducible. It can be used as a method for quality control of the preparation.