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作者将浓度为10菌落数/毫升的大肠杆菌、绿脓杆菌、肺炎杆菌、产气杆菌、硝酸盐阴性杆菌(Mima polymorpha)、金黄色葡萄球菌、表皮葡萄球菌,加至商品0.9%氯化钠溶液、林格氏乳酸盐(RL)、5%葡萄糖林格氏乳酸盐(D5/RL)及5%葡萄糖水溶液(D5/W)中,置于23℃(室温)及37℃下,分别在24、48、72及96小时测定其微生物存活量,并比较这些常见致病菌在四种商品静脉输液中的生长情况。除硝酸盐阴性杆菌在D5/RL中37℃培养24小时内细菌数减少外,革兰氏阴性杆菌在0.9%NaCl,RL及D5/RL中23℃和37℃培养菌落数均有增加。这类细菌在37℃培养一般繁殖较快,常在24小时增加两个对数值,但在23℃培养48小时亦能达到与37℃培养时相同的繁殖水平。在某些情况
The authors added E. coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Aerobacter aerogenes, Mima polymorpha, Staphylococcus aureus, Staphylococcus epidermidis at a concentration of 10 colonies per milliliter to commercial 0.9% sodium chloride (R), 5% glucose Ringer’s lactate (D5 / RL) and 5% dextrose in water (D5 / W) at 23 ° C The microbial viability was measured at 24, 48, 72 and 96 hours respectively, and the growth of these common pathogenic bacteria in four commercial intravenous fluids was compared. The number of colonies of Gram-negative bacilli increased at 23 ℃ and 37 ℃ in 0.9% NaCl, RL and D5 / RL, except that the number of bacteria decreased after nitrate-negative bacterium was cultured in D5 / RL at 37 ℃ for 24 hours. This type of bacteria generally grows faster at 37 ° C, with two log increases often at 24 hours, but at 23 ° C for 48 hours the same level of reproduction as at 37 ° C is achieved. In some cases