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Background The blood vessels of a transplanted organ are the interface between donor and recipient.Theendothelium in the blood vessels is thought to be the major target for graft rejection.Endothelial cells of a transplantedorgan can be of recipient origin after transplantation.In this study,we tested whether endothelial chimerism correlatedwith the graft rejection and cold ischemia.Methods We studied the biopsy samples from 34 renal transplants of female recipients who received the kidney from amale donor for the presence of endothelial cells of recipient origin.We examined the tissue sections of renal biopsysamples by fluorescence in situ hybridization (FISH) for the presence of endothelial cells containing two X chromosomesusing a biotinylated Y chromosome probe and digoxigenin labelled X chromosome probe,and then analyzed therelationship between the endothelial cell chimerism and the rejection and cold ischemia.Results Endothelial chimerism was common and irrespective of rejections (P>O.05).The cold ischemic time ofchimerism group was longer than no chimerism group ((14.83±4.03) hours vs (11.27±3.87) hours,P<0.05).Conclusions There is no correlation between the percentage of recipient endothelial cells in vascular endothelial cellsand the type of graft rejection.The endothelium damaged by ischemic injury might be repaired by the endothelial cellsfrom the recipient.
Background The blood vessels of a transplanted organ are the interface between donor and recipient. Orndothelium in the blood vessels is thought to be the major target for graft rejection. Endothelial cells of a transplantedorgan can be of recipient origin after transplantation. In this study, we tested whether endothelial chimerism correlated with the graft rejection and cold ischemia. Methods We studied the biopsy samples from 34 renal transplants of female recipients who received the kidney from amale donor for the presence of endothelial cells of recipient origin. We examined the tissue sections of renal biopsysamples by fluorescence in situ hybridization (FISH) for the presence of endothelial cells containing two X chromosomes using a biotinylated Y chromosome probe and digoxigenin labeled X chromosome probe, and then analyzed therelationshipbetween the endothelial cell chimerism and the rejection and cold ischemia. Results endothelial chimerism was common and irrespective of rejections (P> 0.05). The cold ischemic time of chimerism group was longer than no chimerism group ((14.83 ± 4.03) hours vs (11.27 ± 3.87) hours, P <0.05) .Conclusions There is no correlation between the percentage of recipient endothelial cells in vascular endothelial cells and the type of graft rejection. the endothelium damaged by ischemic injury might be repaired by the endothelial cells from the recipient.