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小白蛋白 (PV)神经元作为杏仁核簇基底外侧核 (BL)中局部神经环路成分 ,对杏仁核的情绪、学习和记忆过程等机能发挥重要作用。为探讨 BL中 PV中间神经元的突触形成状态 ,本研究用抗 PV抗体标示 PV神经元 ,以抗多巴胺 (DA)抗体标示多巴胺能轴突及末梢作为传入纤维的标志 ,对大鼠杏仁核做了免疫电镜双标记研究。结果表明 ,突触主要见于 PV免疫阳性神经元的树突结构上 ,包括从树突干到中间及小型树突的各级分支。其中 68%的突触由未标记的轴突终末形成 ,3 2 %分别由 DA(2 1% )和 PV(11% )免疫阳性轴突末梢形成。 PV免疫阳性神经元与未标记末梢所形成的突触大多数是对称性的 ,仅少数为非对称性。这些非对称性突触见于 PV神经元的树突小棘和连续性突触 ,即一个未标记轴突末梢与另一个未标记轴突末梢形成对称性突触 ,后者又与 PV免疫阳性神经元树突形成非对称性突触。 DA和 PV免疫阳性神经元轴突终末与 PV免疫阳性神经元树突之间的突触全部是对称性的。以上结果表明 ,大鼠杏仁核 BL 的 PV中间神经元受非对称性突触所构成的包括多巴胺系统在内的抑制性神经网络支配
Small albumin (PV) neurons, as local neural loop components in the basolateral nucleus (BL) of the amygdala cluster, play an important role in the function of the amygdala in emotion, learning and memory processes. In order to investigate the synapse formation status of PV interneurons in BL, PV neurons were labeled with anti-PV antibody. The anti-dopamine (DA) antibody labeled dopaminergic axons and terminals as markers of afferent fibers, Nuclear immunoelectron microscopy double marker study. The results show that synapses are mainly found on the dendritic structures of PV-immunoreactive neurons, including branches from the dendrites to the middle and small dendrites. 68% of the synapses were formed by unlabeled axon terminals and 32% were formed by DA (21%) and PV (11%) immunopositive axon terminals, respectively. Most of the synapses formed by PV-immunopositive neurons and untagged terminals are symmetrical, with only a minority being asymmetric. These asymmetric synapses are found in small dendritic spines and contiguous synapses of PV neurons, ie, one unmarked axon tip forms symmetrical synapses with another unlabeled axon tip, which in turn is associated with the PV immunoreactive nerve Element dendrites form asymmetric synapses. Synapses between axon terminals of DA and PV immunopositive neurons and PV immunopositive neurons are all symmetrical. The above results show that the PV intermediate neurons in the rat amygdala BL are dominated by inhibitory neural networks including asymmetric synapses, including the dopamine system