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[目的]探讨清热化湿益气活血方对幽门螺杆菌(Hp)相关性胃病的作用机制。[方法]70只C57BL/6小鼠分为正常组,模型组,清热化湿益气活血方(中药)高剂量组、中剂量组、低剂量组,西药三联组,中西药联用组,采用氨苄青霉素加Hp灌胃的方法制作Hp感染小鼠模型。除正常组不给药外,其他各组分别于造模后4周给予0.85%氯化钠,高、中、低剂量中药煎剂,奥美拉唑、克拉霉素、甲硝唑混悬液,中剂量中药煎剂加奥美拉唑、克拉霉素、甲硝唑混悬液灌胃给药,连续给药2周。2周后观察小鼠血清一氧化氮(NO)变化情况。[结果]模型组与正常组比较差异有统计学意义(P<0.01);各治疗组小鼠血清NO值与模型组比较均改善明显(P<0.05),与正常组比较差异无统计学意义(P>0.05);中药各治疗组与西药三联组比较P>0.05;中西药联用组与西药三联组比较差异有统计学意义(P<0.05);中药治疗组随剂量的增高而有改善,但无统计学意义(P>0.05)。[结论]清热化湿益气活血方可调节Hp感染小鼠的血清NO至生理水平。
[Objective] To explore the mechanism of Qingre Huashi Yiqi Huoxue Decoction on Helicobacter pylori (Hp)-related gastropathy. [Methods] 70 C57BL/6 mice were divided into normal group, model group, Qingre Huashi Yiqi Huoxue Recipe (Chinese medicine) high-dose group, middle-dose group, low-dose group, western medicine triple group, and Chinese and western drug combination group. A mouse model of Hp infection was made by ampicillin plus Hp gavage. Except for the normal group, no other groups were given 0.85% sodium chloride, high, medium, and low doses of Chinese medicine decoction, omeprazole, clarithromycin, and metronidazole suspension 4 weeks after modeling. The medium-dose Chinese medicinal decoction plus omeprazole, clarithromycin, and metronidazole suspension were administered intragastrically for 2 weeks. After 2 weeks, the changes of serum nitric oxide (NO) were observed. [Results] The difference between the model group and the normal group was statistically significant (P<0.01). The serum NO value of each treatment group was significantly improved compared with the model group (P<0.05), and there was no significant difference between the model group and the normal group. (P>0.05); Chinese medicine treatment group and Western medicine triple group P> 0.05; Chinese and Western medicine combined group and Western medicine triple group difference was statistically significant (P <0.05); Chinese medicine treatment group with the dose increased and improved , but not statistically significant (P>0.05). [Conclusion] Qingre Huashi Yiqi Huoxue Recipe can regulate serum NO level in Hp-infected mice to physiological level.