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目的探讨肾癌干细胞端粒酶活性的改变。方法利用无血清悬浮培养的方法培养富集肾癌干细胞,流式细胞分析技术检测其CD133的表达,用免疫磁珠分选系统分离CD133+及CD133-细胞,以正常肾组织细胞作为阴性对照,采用TRAP实时定量的方法分别检测肾癌CD133+及CD133-细胞端粒酶活性。结果 CD133+细胞和CD133-端粒酶活性分别为(20.54±2.45)和(20.69±2.53)Ct值,而正常肾组织没有检测出端粒酶活性(P<0.05)。结论肾癌干细胞端粒酶活性明显增高。
Objective To investigate the changes of telomerase activity in renal carcinoma stem cells. Methods Kidney cancer stem cells were cultured and enriched by serum-free suspension culture. Flow cytometry was used to detect the expression of CD133. CD133 + and CD133- cells were isolated by immunomagnetic bead sorting system. Normal renal cells were used as negative control. TRAP real-time quantitative detection of renal cell carcinoma CD133 + and CD133-telomerase activity. Results The CD133 + cells and CD133-telomerase activity were (20.54 ± 2.45) and (20.69 ± 2.53) Ct, respectively, while no detectable telomerase activity was found in normal kidney tissue (P <0.05). Conclusion The telomerase activity of renal carcinoma stem cells was significantly increased.