论文部分内容阅读
目的探讨漏声表面波生物传感器检测单核苷酸多态性(single nucleotide polymorphism,SNP)的方法。方法分别在漏声表面波生物传感器反应体系加入2条仅存在一个碱基不同(A/G)的靶序列,与固定的探针杂交。利用酶生物信号放大系统,结合单碱基延伸技术,观察其杂交信号的改变。结果靶位点为完全匹配的A时,相位下降(6.47°±0.42°);而靶位点为错配的G时,相位下降仅为(0.77°±0.25°),两者差异有统计学意义(P<0.01)。结论成功建立了漏声表面波生物传感器检测SNP的新方法。
Objective To explore the method of detecting single nucleotide polymorphism (SNP) by the leaky surface acoustic wave biosensor. Methods Two target sequences with only one base difference (A / G) were added to the SAW biosensor reaction system respectively and hybridized with immobilized probes. The use of enzyme biological signal amplification system, combined with single base extension technology to observe the changes in hybridization signals. Results When the target site was completely matched A, the phase decreased (6.47 ° ± 0.42 °). When the target site was mismatched G, the phase decrease was only (0.77 ° ± 0.25 °), and the difference was statistically significant Significance (P <0.01). Conclusion A new method to detect SNPs by the surface acoustic wave bio-sensor has been established successfully.