Expression of HLA-DR antigen in hepatocellular carcinoma and its up-regulation by interferon

• 作 者: 杨连君 隋延仿
• 出 处: Journal of Medical Colleges of PLA
• 发表日期: 2001年04期
• 关键词: interferon macrophages silane

Objective:To observetheexpressionof humanleukocyticantigenDR(HLA-DR)inprimaryhepatocellular carcinoma(HCC)anditsup-regulationby interferon(IFN).Methods:The expressionof HLA-DRin46specimensof humanHCCtissues,4humanHCCcelllines(SMMC-7721,HCC-9204,BEL-7402andHHCC)anda humanhepatocyte celllineQZGwas respectivelydetectedby immunohistochemicalABCstainingandflowcytometry.Theexpressionof HLA-DRinthe5celllineswasdetectedby ELISAbeforeandafterthecellsweretreatedwithIFN-γor IFN-α.Results:Eighteenoutof46HCCtissues(39.1%)expressedHLA-DR,whereasallthenormallivertissuesimmediatelyadjacentto HCCtissueswereHLA-DR-negative.No obviousHLA-DR-positivestainingwas foundin allthe5celllines.The expressionof HLA-DRwas up-regulatedin allthe5celllinesafterIFN-γor IFN-αtreatment.The up-regulationof HLA-DRinQZGcellswaslessobviousthanthatinHCCcelllines.Theeffectof IFN-γwasmoresignificantthanthatof IFN-α.Conclusion:HCCtissuescanexpressHLA-DRto someextent,butHCCcelllinesdo notexpressdetectable HLA-DR.IFNcanup-regulateHLA-DRexpressioninHCCcells.

Objective: To observe the expression of human leukocyticantigen DR (HLA-DR) in primary hepatocellular carcinoma (HCC) andits up-regulation by interferon (IFN). Methods: The expression of HLA-DRin46 species of human HCC tissue, 4 human HCC cell lines (SMMC-7721, HCC-9204, BEL-7402 and HHCC) anda human hepatocyte cell line QZGwas respectively detectedby immunohistochemicalABCstainingandflowcytometry.Theexpressionof HLA-DRinthe5celllineswasdetectedby ELISAbeforeandafterthecellsweretreatedwithIFN-γor IFN-α.Results: Eighteenoutof46HCCtissues (39.1%) expressedHLA-DR, whereasallthenormallivertissuesimmediatelyadjacentto HCCtissueswereHLA-DR-negative.No obviousHLA-DR-positivestainingwas foundin allthe5celllines.The expressionof HLA-DRwas up-regulatedin allthe5celllinesafterIFN- Γor IFN-α treatment. The up-regulationof HLA-DRinQZGcellswaslessobviousthanthatinHCCcelllines.TheeffectofIFN-γwasmoresignificantthanthatofIFN-α.Conclusion:HCCtissuescanexpressHLA-DRtosomeextent, butHCCcelllinesdo notexpressdetectable HLA-DR.IFNcanup-re gulateHLA-DRexpressioninHCCcells.