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目的:探讨CXC趋化因子受体3(CXCR3)及其配体IP-10和Mig在大鼠肝脏缺血/再灌注(I/R)损伤中的表达及作用.方法:32只Wistar大鼠随机分成4组,每组8只.即假手术组,部分肝脏缺血再灌注6,12和24 h组.应用酶联免疫吸附实验(ELISA)法检测肝组织肿瘤坏死因子(TNF)-α水平.应用半定量聚合酶链式反应(RT-PCR)法测定肝组织CXCR3及其配体IP-10,Mig mRNA的表达.同时检测血清丙氨酸转氨酶(ALT)及天冬氨酸转氨酶(AST)的含量.结果:假手术组肝组织中CXCR3,IP-10,Mig mRNA低表达.缺血再灌注各组肝组织中CXCR3和IP-10 mRNA表达水平均明显高于假手术组(CXCR3:0.925±0.109,0.786±0.074,0.606±0.082 vs 0.125±0.028,均P<0.01;IP-10:0.863±0.091,0.680±0.075,0.543±0.284 vs 0.128±0.027,均P<0.01),6 h组高于12 h组(P<0.01),12h组与24h组无明显差别(P>0.05).Mig mRNA水平较假手术组相比,无显著差异(P>0.05).缺血再灌注各组TNF-α水平较假手术组明显升高(154.88±14-35 ng/L,258.88±13.73 ng/L,182.87±10.95 ng/L vs 23.63±4.00 ng/L,均P<0.01),再灌注12 h达高峰.结论:CXCR3及其配体IP-10在肝脏缺血再灌注早期表达上调,在缺血再灌注损伤中起重要的作用.
Objective: To investigate the expression of CXCR3 and its ligands IP-10 and Mig in liver ischemia / reperfusion (I / R) injury in rats.Methods: 32 Wistar rats The rabbits were randomly divided into 4 groups with 8 rats in each group, sham-operation group, 6th, 12th and 24th hour groups of ischemia / reperfusion of hepatic tissue.Serum levels of tumor necrosis factor-α (TNF-α) were detected by enzyme linked immunosorbent assay (ELISA) Level.The expression of CXCR3 and its ligand IP-10 and Mig mRNA in liver tissues were detected by semi-quantitative polymerase chain reaction (RT-PCR) method.Serum alanine aminotransferase (ALT) and aspartate aminotransferase AST) in the liver tissue of sham operation group.Results: The expression of CXCR3, IP-10 and Mig mRNA in the liver tissue of the sham operation group was low.The expression of CXCR3 and IP-10 mRNA in the liver tissue of ischemia-reperfusion group were significantly higher than that of the sham operation group (CXCR3 : 0.925 ± 0.109,0.786 ± 0.074,0.606 ± 0.082 vs 0.125 ± 0.028, all P <0.01; IP-10: 0.863 ± 0.091,0.680 ± 0.075,0.543 ± 0.284 vs 0.128 ± 0.027, all P <0.01) (P <0.01), while there was no significant difference between 12h and 24h groups (P> 0.05), while there was no significant difference between Mig mRNA and sham-operated group (P> 0.05) TNF-α levels were significantly higher than the sham operation group (154.88 P <0.01), and peaked at 12 h after reperfusion.Conclusion: CXCR3 and its ligand IP- 10 is up-regulated in the early phase of liver ischemia-reperfusion and plays an important role in ischemia-reperfusion injury.