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目的：评价ＰＣＲ扩增ＩＳ６１１０检测结核分枝杆菌复合群的特异性和可靠性。方法：用Ｂａｃｔｅｃ培养法对４５个可疑肺结核病人痰标本进行分枝杆菌检测；用四对分别针对ＩＳ６１１０不同区域引物和一对分枝杆菌属特异的１６ＳｒＲＮＡ基因引物对这４５个痰标本及其液体培养物和２７株已知分枝杆菌株进行ＰＣＲ扩增检测。结果：所有分枝杆菌均扩增出了１６ＳｒＲＮＡ基因片段，结核分枝杆菌复合群均扩增出了ＩＳ６１１０的四个不同区域片段，非典型分枝杆菌均未扩增出ＩＳ６１１０片段，但存在假阳性和假阴性。结论：ＰＣＲ扩增ＩＳ６１１０检测结核分枝杆菌复合群具有特异性，但其结果的可靠性差
Objective: To evaluate the specificity and reliability of PCR amplification of IS6110 in detection of Mycobacterium tuberculosis complex. Methods: Mycobacterium tuberculosis was detected in 45 sputum specimens of suspected pulmonary tuberculosis patients by Bactec culture method. Four pairs of 16S rRNA gene primers specific to different regions of IS6110 and a pair of mycobacteria were used for the detection of 45 sputum specimens and their liquid Cultures and 27 strains of known mycobacteria were PCR amplified. RESULTS: 16S rRNA gene fragments were amplified from all mycobacteria, and four different regions of IS6110 were amplified from Mycobacterium tuberculosis complex. None of the atypical mycobacteria amplified IS6110 fragments, but there were fake Positive and false negative. Conclusion: PCR amplification of IS6110 is specific for the detection of Mycobacterium tuberculosis complex, but the reliability of the results is poor