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本文研究了新的催化显色反应测定痕量硒.方法是在pH2.4的磷酸—磷酸二氢钾介质中,硒(Ⅳ)催化氯酸钾氧化对溴苯肼生成偶氮离子,继而与H-酸偶合成红色的偶氮染料.在一定的硒(Ⅳ)浓度范围内,红色偶氮化合物在520nm处的吸光度与硒(Ⅳ)的量成正比.其线性范围为1.2～12ng/ml,表观摩尔吸光系数达3.65×10~6L·mol~(-1)·cm~(-1),已满意地用于人发样品中痕量硒的测定,并证实癌症病人发样中的硒含量明显低于正常人.
In this paper, a new catalytic chromogenic reaction was developed for the determination of trace selenium by selenium (Ⅳ) -catalyzed oxidation of p-bromophenylhydrazine by potassium chlorate to form azo ions in pH2.4 phosphoric acid-potassium dihydrogen phosphate medium, followed by coupling with H- Acid couple red dye azo.At a certain concentration of selenium (Ⅳ), the absorbance of red azo compounds at 520nm and selenium (Ⅳ) is proportional to the amount of its linear range of 1.2 ~ 12ng / ml, the table The molar extinction coefficient of 3.65 × 10 ~ 6L · mol ~ (-1) · cm ~ (-1) has been used satisfactorily for the determination of trace selenium in human hair samples and confirms the selenium content of hair samples from cancer patients Obviously lower than normal people.