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菜豆(Phaseolus vulgaris)是古巴最重要的粮食作物之一。2012年1月,古巴马坦萨斯(Matanzas)、玛雅贝克(Mayabeque)、阿尔特米萨(Mayabeque)等地的菜豆多个栽培种萎蔫并死亡,发病率为21%~85%。植株感病初期,叶片萎蔫,豆荚及茎上出现水渍状病斑,随后形成白色菌丝体,病斑为圆形或不规则状,直径约5~6mm。从病株取样,经表面消毒、蒸馏水冲洗后转移至改良的PDA培养基,于22℃条件下培养,均长出蓬松的白色菌丝体及黑色菌核。经形态学特征鉴定病原菌为核盘菌(Sclerotiniasclerotiorum)。
Phaseolus vulgaris is one of the most important food crops in Cuba. In January 2012, many cultivars of bean varieties such as Matanzas, Mayabeque and Mayabeque in Cuba wilted and died, at a rate of 21% -85%. At the early stage of plant infection, the leaves wilted, watery spots appeared on the pods and stems, and then white mycelia formed. The lesions were round or irregular with a diameter of about 5 to 6 mm. Samples were taken from diseased plants, surface-sterilized, rinsed with distilled water, transferred to modified PDA medium, and cultivated at 22 ° C to grow fluffy white mycelium and black sclerotia. The morphological characteristics of the identified pathogen Sclerotinasclerotiorum.