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目的观察高压氧对大白鼠失血性休克复苏后炎症反应的影响并探讨其作用机制。方法Wistar大鼠随机分为三组:高压氧治疗组、休克组和对照组。建立大鼠失血性休克模型,自体血和生理盐水复苏后应用2.0绝对大气压高压氧治疗,于休克前、休克后、复苏后和复苏后24 h取血检测血红细胞SOD、血浆TNF-α、血浆iNOS值,并进行统计学(one-way ANOVA plus SNK)分析。观察复苏后24 h大鼠肝、肠、肺组织病理改变并进行病理损伤评分。结果复苏后24 h高压氧治疗组血红细胞SOD值高于休克组,高压氧治疗组血浆iNOS值和TNF-α值低于休克组,差异具有统计学意义(P<0.05)。病理损伤评分:高压氧治疗组病理损伤与休克组相比减轻,差异具有统计学意义(P<0.05)。结论高压氧可能通过增强机体清除活性氧和自由基、阻止炎症介质的产生,进而减轻大鼠失血性休克复苏后的炎症反应和组织脏器的病理损伤。
Objective To observe the effect of hyperbaric oxygen on the inflammatory response after hemorrhagic shock resuscitation in rats and its mechanism. Methods Wistar rats were randomly divided into three groups: hyperbaric oxygen therapy group, shock group and control group. The model of hemorrhagic shock in rats was established. After resuscitation with autologous blood and saline, hyperbaric oxygen of 2.0 absolute atmospheric pressure was used. Blood samples were collected before shock, after shock, 24 h after resuscitation and 24 h after resuscitation for determination of SOD, plasma TNF-α, iNOS values and one-way ANOVA plus SNK analysis. The pathological changes of liver, intestine and lung in 24 h after resuscitation were observed and pathological damage scores were observed. Results After 24 h of resuscitation, the level of SOD in erythrocytes was higher in hyperbaric oxygen group than in shock group. The plasma iNOS and TNF-α in hyperbaric oxygen group were lower than those in shock group (P <0.05). Pathological damage score: The hyperbaric oxygen treatment group pathological injury compared with the shock group, the difference was statistically significant (P <0.05). Conclusions Hyperbaric oxygen may reduce the inflammatory reaction and the pathological damage of tissues and organs after hemorrhagic shock resuscitation in rats by enhancing the body’s ability of scavenging reactive oxygen and free radicals and preventing the production of inflammatory mediators.