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目的探讨Gab2在胶质瘤组织中的表达及对胶质瘤大鼠模型MMP-2、MMP-9表达的影响。方法荧光定量PCR检测40例胶质瘤和瘤旁组织中Gab2的m RNA表达;培养C6胶质瘤细胞,分为A:对照组;B:空载体组;C:Gab2-si RNA组,RT-PCR检测3组细胞Gab2的表达;大鼠注射胶质瘤细胞和Gab2-si RNA胶质瘤细胞,构建大鼠模型,检测注射两组细胞1、2、3周后大鼠肿瘤体积和肿瘤强化程度,RT-PCR检测注射两组细胞3周后大鼠MMP-2、MMP-9的m RNA和蛋白表达。结果胶质瘤组Gab2的表达显著高于瘤旁组织(P<0.01);Gab2-si RNA组Gab2蛋白表达显著低于对照组(P<0.01),空载体组Gab2蛋白表达与对照组差异不显著(P>0.05);第2周大鼠的肿瘤体积和强化程度显著高于第1周,第3周显著高于第2周(P<0.01);注射Gab2-si RNA胶质瘤组大鼠的肿瘤体积和肿瘤强化程度显著低于胶质瘤组(P<0.01),Gab2-si RNA胶质瘤组MMP-2、MMP-9的m RNA和蛋白表达显著低于胶质瘤组(P<0.01)。结论 Gab2在胶质瘤组织中高表达,能通过下调MMP-2、MMP-9的表达降低胶质瘤体积和肿瘤强化程度。
Objective To investigate the expression of Gab2 in glioma and its effect on the expression of MMP-2 and MMP-9 in rat glioma model. Methods The m RNA expression of Gab2 in 40 gliomas and adjacent normal tissues was detected by fluorescence quantitative PCR. C6 glioma cells were cultured and divided into A: control group, B: empty vector group, C: Gab2-si RNA group, RT The expression of Gab2 in three groups of cells was detected by PCR. Glioma cells and Gab2-si RNA were injected into rat brain to establish a rat model. The tumor volume and tumor volume The mRNA and protein expression of MMP-2 and MMP-9 in rats were measured by RT-PCR three weeks after injection. Results The expression of Gab2 in glioma group was significantly higher than that in para-tumor group (P <0.01). The expression of Gab2 protein in Gab2-si RNA group was significantly lower than that in control group (P <0.01). The expression of Gab2 protein in empty vector group was not significantly different from that in control group (P> 0.05). The tumor volume and degree of enhancement in the second week were significantly higher than those in the first week and the third week were significantly higher than those in the second week (P <0.01) (P <0.01). The m RNA and protein expressions of MMP-2 and MMP-9 in Gab2-si RNA glioma group were significantly lower than those in glioma group (P <0.01) P <0.01). Conclusions Gab2 is highly expressed in glioma tissues, which can decrease the volume of glioma and the degree of tumor enhancement by down-regulating the expression of MMP-2 and MMP-9.