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背景高血压是最常见的心血管疾病,而高血压实验动物模型在高血压研究中扮演着重要角色。目的建立稳定的甲状腺功能亢进(甲亢)性高血压家兔模型,观察其相关指标的动态变化。方法选用新西兰大耳白家兔,给家兔皮下以0.5mg/(kg·d)的剂量分别注射L甲状腺素(T4)3、6、12和14d,制备不同程度的甲亢性高血压家兔模型,并观察心脏质量指数、体温、心率、收缩压、血浆心房利钠肽(ANP)水平的动态变化。结果 T4注射12d组存活率明显高于14d组。T4注射3d后,各项指标如心脏质量指数、体温、心率、收缩压和血浆ANP水平较正常对照组升高,其增高幅度分别为13.9%、1.4%、33.5%、11.9%和37.5%;T4注射12d组与14d组相比,上述指标无明显差异,表明12d组则进入较稳定的阶段,此时各项指标较正常对照组升高,其增高幅度分别为67.0%、3.3%、57.9%、29.3%和365.9%。单因素相关分析显示,在甲亢性高血压造模过程中心率、收缩压和血浆ANP水平与心脏质量指数之间均呈正相关(心率与心脏质量指数:r=0.927,P<0.01;收缩压与心脏质量指数:r=0.976,P<0.01;血浆ANP水平与心脏质量指数r=0.824,P<0.01)。结论给家兔皮下以0.5mg/(kg·d)的剂量T4注射12d,可获得较稳定的甲亢性高血压家兔实验模型,且在甲亢性高血压造模过程中心率、收缩压和血浆ANP水平与心脏质量指数之间均呈正相关。此造模方法较简便、成功率高、周期较短和经济易行。
Background Hypertension is the most common cardiovascular disease, and animal models of hypertension play an important role in the study of hypertension. Objective To establish a stable hyperthyroidism (hyperthyroidism) rabbit model of hypertension, observe the dynamic changes of related indicators. Methods New Zealand white rabbits were selected and the rabbits were injected subcutaneously with 0.5 mg / (kg · d) L thymosin (T4) for 3, 6, 12 and 14 days respectively to prepare hyperthyroidism hypertensive rabbits The changes of heart mass index, body temperature, heart rate, systolic pressure, and plasma atrial natriuretic peptide (ANP) levels were observed. Results The survival rate of T4 injection 12 d group was significantly higher than that of 14 d group. After 3d injection of T4, indexes such as heart mass index, body temperature, heart rate, systolic blood pressure and plasma ANP level increased 13.9%, 1.4%, 33.5%, 11.9% and 37.5% higher than those of normal control group respectively. There were no significant differences in the above indexes between the 12-day T4 injection and the 14-day group, indicating that the 12-day group entered a more stable stage. At this time, all the indexes increased compared with the control group, and the increase was 67.0%, 3.3%, 57.9 %, 29.3% and 365.9%. Univariate correlation analysis showed that heart rate, systolic blood pressure and plasma ANP levels were positively correlated with cardiac mass index in hyperthyroidism model (heart rate and heart mass index: r = 0.927, P <0.01; systolic blood pressure and Heart mass index: r = 0.976, P <0.01; plasma ANP level and cardiac mass index r = 0.824, P <0.01). CONCLUSIONS: A stable hypertensive rabbit model of hyperthyroidism was obtained in rabbits subcutaneously at a dose of 0.5 mg / (kg · d) for 12 days. The heart rate, systolic pressure and plasma There was a positive correlation between ANP level and cardiac mass index. This modeling method is relatively simple, high success rate, short cycle and economic feasibility.