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目的:研究实验性大鼠胃溃疡期间海马区肠三叶因子(ITF)表达的变化。方法:选择SD大鼠42只,其中实验性胃溃疡36只,正常对照6只,用免疫组织化学染色和RT-PCR方法分别检测大鼠海马ITF蛋白表达量和mRNA转录变化。结果:大鼠海马锥体细胞和颗粒细胞质内,以及海马内部分神经细胞可见ITF免疫反应阳性物质。与正常大鼠比较,实验性大鼠溃疡1 d后海马ITF平均灰度值开始降低,随后逐渐降低,1周后达到最低水平(P<0.01),维持2~4周后开始恢复(P<0.05)。RT-PCR检测表明,大鼠实验性溃疡后1 d开始,海马ITF mRNA转录水平逐渐升高,1周达到最高值,其后开始下降(P<0.05~0.01),4周后接近实验前水平。结论:实验性胃溃疡大鼠海马中ITF高表达可能是溃疡愈合过程神经内分泌调节的重要因素。
Objective: To study the changes of intestinal trefoil factor (ITF) in hippocampus during experimental gastric ulcer in rats. Methods: Forty-two SD rats were selected, including 36 experimental gastric ulcer and 6 normal control. The expression of ITF protein and mRNA transcription in the hippocampus were detected by immunohistochemical staining and RT-PCR. RESULTS: ITF immunoreactive substances were found in the pyramidal cells and granular cytoplasm of the hippocampus, and in some neurons in the hippocampus. Compared with normal rats, the average gray value of ITF in hippocampus began to decrease after 1 d of experimental ulcer in rats, then gradually decreased and reached the lowest level after 1 week (P <0.01), and recovered after 2 to 4 weeks (P < 0.05). The results of RT-PCR showed that the level of ITF mRNA in hippocampus gradually increased from the first day after experimental ulcer in rats to the highest value in one week and then decreased (P <0.05-0.01), and reached the level of pre-experiment 4 weeks later . Conclusion: The high expression of ITF in the hippocampus of experimental gastric ulcer rats may be an important factor in the neuroendocrine regulation of ulcer healing.