论文部分内容阅读
目的评价重组人白细胞介素11(rhIL)对5氟尿嘧啶(5-FU)化疗后大鼠肠结构及肠屏障的影响。方法30只SD大鼠随机分为正常对照组(A组)、化疗模型组(B组)和rhIL-11治疗化疗模型组(C组),每组10只。B、C两组行5-FU75 mg/kg腹腔内注射化疗1 d。C组于化疗开始后3 d天注射rhIL-11 600.μg.kg-1.d-1。化疗前及化疗后第5天分别称空腹体重,实验后第5天测肠结构、肠系膜淋巴结细菌移位、肠道通透性。结果C组体重丢失少于B组,A组体重增加显著多于B、C组(P<0.01)。C组的各项肠结构指标均优于B组(P<0.05)。C、A组的细菌移位率(10%)低于B组的60%(P<0.05)。B组的肠通透性与A、C组相比较有非常显著性差异(P<0.01)。结论rhIL-11对化疗大鼠的肠结构和屏障功能有保护作用。
Objective To evaluate the effect of recombinant human interleukin 11 (rhIL) on intestinal structure and gut barrier in rats after chemotherapy with 5-fluorouracil (5-FU). Methods Thirty SD rats were randomly divided into normal control group (A group), chemotherapy group (B group) and rhIL-11 treatment group (C group), with 10 rats in each group. B, C two groups of 5-FU75 mg / kg intraperitoneal injection of chemotherapy for 1 d. Group C received rhIL-11 600.μg.kg-1.d-1 on the third day after the start of chemotherapy. Before chemotherapy and 5 days after chemotherapy were weighed fasting body weight respectively. On the fifth day after the experiment, intestinal structure, bacterial translocation of mesenteric lymph nodes and intestinal permeability were measured. Results The body weight loss in group C was less than that in group B, and the weight gain in group A was significantly more than that in group B and C (P <0.01). The indexes of intestinal structure in group C were better than those in group B (P <0.05). In group C, the bacterial translocation rate (10%) was lower in group A than in group B (60%, P <0.05). Intestinal permeability in group B was significantly different from that in group A and C (P <0.01). Conclusion rhIL-11 has a protective effect on intestinal structure and barrier function in rats after chemotherapy.