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目的:探讨缺血预处理 (IP)对兔肺缺血 /再灌注 (I/ R)损伤的保护作用。 方法 :将 30只新西兰白兔随机分为对照组 (C组 )、缺血 /再灌注组 (I/ R组 )和缺血预处理组 (IP组 ) ,每组 10只。对比观察各组同侧肺静脉血氧分压 (Pa O2 )、肺湿 /干重比、血清及肺组织超氧化物歧化酶 (SOD)活性、丙二醛 (MDA)、髓过氧化物酶 (MPO)含量和肺组织形态学变化。结果:再灌注后 I/ R组 Pa O2 呈进行性下降 ,尤以再灌注 30 min内明显 ;IP组 Pa O2 、SOD活性和 MPO含量均优于 I/ R组 (P <0 .0 5 ) ,肺湿 /干重比和 MDA含量均低于 I/ R组 (P <0 .0 1) 。结论 :肺的缺血预处理可通过减轻肺毛细血管的通透性 ,提高机体抗氧化自由基的能力 ,减轻 I/ R损伤 ,起到保护肺脏的作用。
Objective: To investigate the protective effect of ischemic preconditioning (IP) on lung ischemia / reperfusion (I / R) injury in rabbits. Methods: Thirty New Zealand white rabbits were randomly divided into control group (C group), ischemia / reperfusion group (I / R group) and ischemic preconditioning group (IP group). PaO2, lung wet / dry weight ratio, serum and lung tissue superoxide dismutase (SOD) activity, malondialdehyde (MDA), myeloperoxidase MPO) content and lung histological changes. Results: PaO2 in I / R group decreased progressively after reperfusion, especially within 30 min reperfusion. PaO2, SOD activity and MPO content in IP group were better than those in I / R group (P <0.05) , Lung wet / dry weight ratio and MDA content were lower than those in I / R group (P <0.01). CONCLUSION: Ischemic preconditioning of the lungs can protect the lungs by reducing the permeability of pulmonary capillaries, increasing the body’s ability to resist free radicals and reducing I / R injury.