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目的探讨抗氧化剂NAC在SAP大鼠模型上对核因子-κB和环氧合酶-2的影响及可能机制。方法雄性SD大鼠36只随机分成假手术组(A组),SAP组(B组),SAPNAC干预组(C组);每组各12只。胆胰管逆行穿刺注射5%牛磺胆酸(1.0 mL/kg)建立SAP模型。C组造模前1 h腹腔注射NAC 0.05 mg/kg。术后6 h检测各组血清淀粉酶活性,IL-1β浓度,胰腺组织COX-2和NF-κB的表达。光镜观察胰腺病理变化,对损伤程度进行评分。结果B组和C组淀粉酶活性和IL-1β浓度显著高于A组(P<0.01),B组和C组IL-1β浓度无差异(P>0.05)。COX-2与NF-κB在A组胰腺组织无表达,在B,C组均有阳性表达,B组阳性细胞比例较C组高(P<0.05)。病理评分提示B,C组水肿、炎症、坏死以及出血均有显著升高,C组水肿和坏死评分较B组降低(P<0.05)。结论SAP时,胰腺组织可以出现COX-2与NF-κB的高表达,抗氧化剂NAC能够抑制NF-κB和COX-2在胰腺组织的表达,减轻胰腺炎的严重程度。
Objective To investigate the effect of antioxidant NAC on nuclear factor-κB and cyclooxygenase-2 in a rat model of SAP and its possible mechanism. Methods Thirty - six male Sprague Dawley rats were randomly divided into sham operation group (A group), SAP group (B group) and SAPNAC intervention group (C group). The biliary and pancreatic duct retrograde puncture injection of 5% taurocholic acid (1.0 mL / kg) SAP model. Group C was intraperitoneally injected with NAC 0.05 mg / kg 1 h before modeling. Serum amylase activity, IL-1β concentration, expression of COX-2 and NF-κB in pancreatic tissue were measured at 6 h after operation. Light microscopy pathological changes in the pancreas, the extent of injury were scored. Results The amylase activity and the concentration of IL-1β in group B and C were significantly higher than those in group A (P <0.01). There was no difference in IL-1β between group B and C (P> 0.05). There was no expression of COX-2 and NF-κB in the pancreatic tissue in group A, but positive expression in group B and C, and the percentage of positive cells in group B was higher than that in group C (P <0.05). The pathological score indicated that the edema, inflammation, necrosis and hemorrhage of group B and C were significantly increased, while the score of edema and necrosis of group C was lower than that of group B (P <0.05). Conclusions The expression of COX-2 and NF-κB in pancreatic tissue may be high in SAP. Antioxidant NAC can inhibit the expression of NF-κB and COX-2 in pancreatic tissue and reduce the severity of pancreatitis.