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为了研究LHRH(促黄体激素释放激素)对雄性大鼠垂体细胞分泌LH(促黄体激素)是否有自身激发作用,我们用不同幅度(1×10~(-10)～1×10~(-8) mol/L)和不同频率(1～4脉冲/小时)的LHRH脉冲刺激灌流的SD雄性大鼠垂体前叶细胞,观察了在灌流系统中细胞LH的分泌反应。结果表明,在高幅度(1×10~(-9)mol/L或更大)和高频率(3脉冲或更高)LHRH脉冲作用下,灌流的垂体细胞可表现自身激发作用,即在LHRH刺激一定时间后,同样的LHRH刺激产生更大的LH释放。但是,低幅度(1×10~(10)mol/L)LHRH即使在高频率下也不能显著地改变LH反应性。上述实验说明:离体成年雄性大鼠垂体前叶细胞存在对LHRH的自身激发作用,这种自身激发作用的产生主要依赖于LHRH脉冲的幅度和频率。
In order to investigate whether LHRH exerts its own stimulatory effects on the secretion of LH by the pituitary cells of male rats, we observed the effects of LHRH on the secretion of LH (luteinizing hormone) ) mol / L) and LHRH pulses of different frequencies (1 ~ 4 pulses / h) were used to stimulate the anterior pituitary cells of SD male rats. The secretion of LH in the perfusion system was observed. The results showed that the perfusion of pituitary cells could manifest self-excitation under the action of high amplitude (1 × 10 -9 mol / L or more) and high frequency (3 pulses or more) LHRH pulses, ie, LHRH After a certain period of stimulation, the same LHRH stimulation produced greater LH release. However, low amplitude (1 × 10 ~ (10) mol / L) LHRH can not significantly change LH reactivity even at high frequency. The above experiment shows that the anterior pituitary cells of isolated adult male rats present the self-excitation of LHRH, and the self-excitation mainly depends on the amplitude and frequency of LHRH pulses.