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目的:探讨胰岛素样生长因子结合蛋白2(IGFBP-2)在卵巢肿瘤组织中的表达及其与卵巢癌临床病理学特征的关系。方法:选择正常卵巢组织(10例)和卵巢肿瘤组织(卵巢良性肿瘤12例,卵巢交界性肿瘤10例,卵巢癌26例,包括中度和高度分化者12例,低度分化者14例),采用逆转录-聚合酶链反应(RT-PCR)方法检测卵巢组织IGFBP-2基因表达情况,Western blotting检测卵巢肿瘤组织IGFBP-2蛋白表达情况。结果:RT-PCR和Western blotting结果显示IGFBP-2在卵巢癌的表达明显升高(P<0.05),且随着卵巢癌组织分化程度的降低IGFBP-2的表达增强。结论:卵巢癌患者IGFBP-2表达与临床病理学特征有明显的相关性,可作为卵巢癌患者预后判断的标志。
Objective: To investigate the expression of insulin-like growth factor binding protein 2 (IGFBP-2) in ovarian cancer and its relationship with the clinicopathological features of ovarian cancer. Methods: Normal ovarian tissue (10 cases) and ovarian tumor (12 cases of ovarian benign tumor, 10 cases of ovarian borderline tumor and 26 cases of ovarian cancer, including 12 cases of moderate and highly differentiated, 14 cases of poorly differentiated) The expression of IGFBP-2 gene in ovarian tissue was detected by reverse transcription-polymerase chain reaction (RT-PCR). The protein expression of IGFBP-2 in ovarian tumor tissue was detected by Western blotting. Results: The results of RT-PCR and Western blotting showed that the expression of IGFBP-2 in ovarian cancer was significantly increased (P <0.05), and the expression of IGFBP-2 was enhanced with the differentiation of ovarian cancer. Conclusion: The expression of IGFBP-2 in ovarian cancer patients has obvious correlation with clinicopathological features, which may be used as a marker of prognosis in patients with ovarian cancer.