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以交联聚乙烯醇(CPVA)微球为基质,采用接枝聚合和表面印迹同步技术制备碱性蛋白胰蛋白酶(TRY)分子表面印迹材料,甲基丙烯酰氯与CPVA微球表面的羟基发生快速酯化反应,得到表面含大量可聚合双键甲基丙烯酰基(MAO)的改性微球MAO-CPVA.按一定摩尔比将TRY和单体阴离子单体对苯乙烯磺酸钠(SSS)溶解在水溶液中,加入交联剂N,N’-亚甲基双丙烯酰胺(MBA),MAO-CPVA分散于水介质中,过硫酸铵/亚硫酸氢钠引发体系产生自由基,使包围在TRY周围的单体SSS与MBA在MAO-CPVA表面发生接枝交联聚合,制得TRY表面印迹微球MIP-PSSS/CPVA,对其进行表征,考察了其大分子识别性能.结果表明,MIP-PSSS/CPVA对TRY有优良的亲和性和特异识别选择性,吸附容量达85.9 mg/g,对TRY的选择性系数相对于蛋白溶菌酶LZM达17.52.
Alkaline protein trypsin (TRY) molecular imprinting material was prepared by using cross-linked polyvinyl alcohol (PVA) microspheres as the substrate. The hydroxyl groups on the surface of methacrylic chloride and CPVA microspheres were rapidly Esterification reaction to obtain modified microspheres MAO-CPVA containing a large amount of polymerizable double bond methacryloyl groups (MAO) on the surface.The TRY and monomeric anionic monomer sodium styrene sulfonate (SSS) were dissolved in a certain molar ratio In the aqueous solution, the crosslinking agent N, N’-methylenebisacrylamide (MBA) is added, the MAO-CPVA is dispersed in an aqueous medium, the ammonium persulfate / sodium bisulfite induces a free radical generating system, Surrounding monosomic SSS and MBA were grafted and crosslinked on the surface of MAO-CPVA to obtain MIP-PSSS / CPVA of TRY surface imprinted microspheres, and their macromolecular recognition properties were investigated.The results showed that MIP- PSSS / CPVA has good affinity and specificity for TRY with an adsorption capacity of 85.9 mg / g and a selectivity coefficient for TRY of 17.52 relative to lysozyme LZM.