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Aim: To study the effects of propyl gallate on the interaction of tumor necrosisfactor-α(TNF-α) with its soluble receptor, sTNFR-I. Methods: Interactions between TNF-α and sTNFR-I were analyzed using an IAsys biosensor, sTNFR-Iwas immobilized on the carboxymethyl dextran (CMD) surface of the IAsys biosensor cuvettes, and TNF-α preincubated with different concentrations of propylgallate was added to the cuvettes. The resonant angle shift caused by the bindingbetween TNF-α and sTNFR-I was then recorded. Results: sTNFR-I was immobilized on the CMD surface at a density of 2.76 ng/mm~2. TNF-α then bound theimmobilized sTNFR-I specifically, and propyl gallate was able to enhance thebinding between TNF-α and sTNFR-Iin a dose-dependent manner. Conclusion:The binding between TNF-α and sTNFR-I is one of the targets that propyl gallatecan act on in vivo. The IAsys biosensor offers a new clue as to the study on themechanisms of action of propyl gallate.
Method: Interactions between TNF-α and sTNFR-I were analyzed using an -Iwas immobilized on the carboxymethyl dextran (CMD) surface of the IAsys biosensor cuvettes, and TNF-α preincubated with different concentrations of propylgallate was added to the cuvettes. The resonant angle shift caused by the bindingbetween TNF-α and sTNFR-I was then Recorded. Results: sTNFR-I was immobilized on the CMD surface at a density of 2.76 ng/mm~2. TNF-α then bound theimmobilized sTNFR-I specifically, and propyl gallate was able to enhance the binding between TNF-α and sTNFR- The IAsys biosensor offers a new clue as to the study on theme chanchans of action of propyl gallate. .