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目的 研究转化生长因子 β1(transforminggrowthfactorβ1,TGF β1)对异体角膜上皮所致的外周淋巴细胞亚群CD+ 69及CD+ 2 5活化的影响。方法 采用CD+ 69、CD+ 2 5间接免疫荧光标记技术 ,行流式细胞仪检测及分析CD+ 69、CD+ 2 5的表达率。结果 培养的异体角膜上皮细胞与外周血共孵育 3h ,测得CD+ 69及CD+ 2 5表达率均较对照组有显著升高 ;经TGF β1处理后 ,CD+ 69及CD+ 2 5表达率明显受到抑制。结论 TGF β1对异体角膜上皮细胞所致的T淋巴细胞早期活化分子CD+ 69及CD+ 2 5的表达有明显的抑制作用。
Objective To investigate the effect of transforming growth factor β1 (TGFβ1) on the activation of CD + 69 and CD + 2 in peripheral lymphocytes induced by allogeneic corneal epithelium. Methods The expression of CD + 69 and CD + 25 was detected by flow cytometry with indirect immunofluorescence labeling (CD + 69 and CD + 25). Results The cultured corneal epithelial cells were co-incubated with peripheral blood for 3 h. The expression of CD + 69 and CD + 25 was significantly higher than that of the control group. The expression of CD + 69 and CD + 25 was significantly inhibited by TGF- . Conclusion TGF β1 can significantly inhibit the expression of CD + 69 and CD + 25 in early stage of T lymphocytes induced by allogeneic corneal epithelial cells.