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目的探讨类风湿性关节炎患者(rheumatoid arthritis,RA)关节液中巨噬细胞移动抑制因子(macrophage migration inhibitory factor,MIF)与血管内皮生长因子(vascular endothelial growth factor,VEGF)、磷脂酶A2(phospholipase A2,PLA2)的关系。方法 RA患者30例为RA组,体检健康者30例为对照组,2组采用ELISA法检测患者关节液中MIF、VEGF及PLA2水平,采用RT-PCR检测关节液中MIF、VEGF及PLA2基因表达情况。结果 RA组MIF、VEGF和PLA2水平明显高于对照组(P<0.05);RA组MIF、VEGF、PLA2表达水平在晨僵时间、关节肿胀个数及有无手X线异常改变上差异有统计学意义(P<0.05),在有无皮下结节、类风湿因子及血沉水平比较差异无统计学意义(P>0.05);RA组MIF高表达者关节液VEGF、PLA2基因表达较MIF低表达者明显增高(P<0.05)。结论 RA患者关节液中MIF水平明显增高,并与VEGF、PLA2表达密切相关。
Objective To investigate the relationship between macrophage migration inhibitory factor (MIF) and vascular endothelial growth factor (VEGF), phospholipase A2 (RA) in rheumatoid arthritis (RA) A2, PLA2) relationship. Methods Thirty patients with RA were selected as RA group and 30 healthy controls as control group. The levels of MIF, VEGF and PLA2 in synovial fluid were detected by ELISA in two groups. The expressions of MIF, VEGF and PLA2 in synovial fluid were detected by RT-PCR Happening. Results The levels of MIF, VEGF and PLA2 in RA group were significantly higher than those in control group (P <0.05). There were statistically significant differences in the expressions of MIF, VEGF and PLA2 in RA group between morning stiffness, joint swelling and the presence of abnormal X-ray (P <0.05). There was no significant difference in the presence or absence of subcutaneous nodules, rheumatoid factor and erythrocyte sedimentation rate (P> 0.05). The expression of VEGF and PLA2 in synovial fluid of RA patients with low MIF expression was lower than that of MIF Were significantly higher (P <0.05). Conclusions MIF levels in synovial fluid of patients with RA are significantly increased, and are closely related to the expression of VEGF and PLA2.