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一、试验材料和方法在本试验中使用的材料是椰子种子园中长了12—13个月的椰子。把椰子胚切下,然后用5％次氯酸钙消毒10分钟,才放进培养基。培养阶段如下: 1.第1阶段,把胚置于液体培养基中培养,用每分钟60转的速度搅拌,每天12小时,历时8周。然后将胚移到琼脂培养基中
First, the test materials and methods The materials used in this experiment is coconut seed garden in the 12-13 months long coconut. The coconut embryos are cut and then sterilized with 5% calcium hypochlorite for 10 minutes before being put in the medium. The cultivation stages are as follows: 1. In the first stage, the embryos are cultured in liquid medium and agitated at 60 revolutions per minute for 12 hours for 8 weeks. The embryos are then transferred to agar medium