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神经干细胞(NSCs)具有替代及修复受损神经组织的作用,研究发现能够通过调控NSCs内环境诱导其增殖分化,重塑神经功能,从而修复脑卒中神经病变等神经系统损伤。本实验研究了蒙药白脉散有效成分组(BMECG)激活NSCs增殖的药理学作用。通过建立体内外2种损伤模型,模拟脑卒中神经病变,分别利用流式细胞术、行为学检测、Nestin免疫组化等方法研究BMECG的脑保护作用机制。结果表明,BMECG能够显著提高体外培养NSCs增殖能力,有助于小鼠的行为学功能恢复,明显改善模型小鼠大脑皮层NSCs增殖能力。因此BMECG直接针对NSCs增殖存活能力发挥作用,可能是治疗脑卒中神经病变的机制之一,该药物具有重要的研究意义和广阔的应用前景。
Neural stem cells (NSCs) have the function of replacing and repairing damaged nerve tissue. It has been found that neural stem cells (NSCs) can repair neurological damage such as neuropathies by regulating the environment of NSCs to induce their proliferation, differentiation and remodeling of nerve function. This experiment studied the pharmacological effects of Mongolian medicine Baimai San active ingredient group (BMECG) on the proliferation of NSCs. By establishing two kinds of injury models in vivo and in vitro, the neuropathies of stroke were simulated, and the mechanism of brain protective effect of BMECG was studied by flow cytometry, behavioral test and Nestin immunohistochemistry respectively. The results showed that BMECG can significantly enhance the proliferation of NSCs cultured in vitro, which can help the mice to recover their behavioral functions and significantly improve the proliferation of NSCs in the model mice. Therefore, BMECG plays a direct role in the proliferation and survival of NSCs, which may be one of the mechanisms for the treatment of neuropathies. This drug has important research significance and broad application prospects.