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目的 研究樟科药用植物乌药 [Linderaaggregata (Sims)Kosterm .]的抗HIV 1整合酶的活性成分。 方法 采用 6 0 %丙酮冷浸提取 ,通过反复SephadexLH 2 0和MCI gelCHP 2 0P柱色谱从乌药茎中分离缩合鞣质类化合物 ,通过波谱解析和理化常数进行结构鉴定 ,采用MIA法进行抗HIV 1整合酶活性筛选。结果 分离得到 3个寡聚缩合鞣质类化合物 :二倍体 pro cyanidinB1(LA 1) ,三倍体cinnamtaninB1(LA 2 )和四倍体cinnamtanninB2 (LA 3)。活性筛选结果表明 ,寡聚缩合鞣质具有抗HIV 1整合酶的活性。结论 寡聚缩合鞣质是乌药茎抗HIV 1整合酶的主要活性成分 ,四倍体cinnamtanninB2 (3)为种内首次分离得到
Objective To study the anti-HIV-1 integrase active ingredients of Lindera aggregata (Sims) Kosterm. Methods Condensed tannins were isolated from stems of Syringnia obliya by repeated SephadexLH 2 0 and MCI gelCHP 20P column chromatography using 60% acetone cold leaching. The structures were identified by spectral analysis and physicochemical constants, and anti-HIV was performed by MIA method. 1 Screening for integrase activity. Results Three oligomeric condensed tannin compounds were isolated: diploid pro cyanidin B1 (LA 1), triploid cinnamtanin B1 (LA 2) and tetraploid cinnamtannin B2 (LA 3). The activity screening results showed that the oligomeric condensed tannin had anti-HIV 1 integrase activity. Conclusion The oligomeric condensed tannin is the main active component of the anti-HIV 1 integrase of the stem of Syringa obliquus. The tetraploid cinnamtannin B2 (3) was isolated for the first time in the species.