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为了探讨中枢神经系统对咽肌前运动神经元的神经调控机制,用免疫组织化学方法研究了假狂犬病毒(PRV)注射咽肌后跨突触标记细胞在脑中的分布。咽肌注射PRV后,脑内PRV标记细胞出现的部位随着动物存活时间的延长而增多。咽肌注射PRV后48h,仅在疑核半致密部中出现阳性标记细胞。存活56h后,标记细胞可见于孤束核的中介亚核、中间亚核及吻内侧亚核。存活62~72h后,在延髓的中缝核群、三叉旁核、外侧网状核,脑桥的蓝班、臂旁核、A5细胞群等部位可见大量标记细胞。存活80~96h后,延髓和脑桥中上述部位的标记细胞更为密集,并在三叉神经脊束核及Barington核等出现标记细胞。中脑的中央灰质和中脑深核;丘脑下部的视前区、室旁核、外侧区、弓状核、连合前核;丘脑的室旁核和外侧缰核;端脑的外侧隔核、终纹床核、杏仁核等部位可见大量阳性细胞。存活102h的动物,标记细胞的分布部位和存活96h者类似,但数量更多。且在皮层的边缘前区、内侧和外侧中央前区等出现较多标记细胞。推测脑内许多部位与咽肌前运动神经元的神经调控有关
To investigate the neuromodulatory mechanism of the central nervous system to the motoneurons of the pharyngeal motoneuron, the distribution of trans-synaptic markers in the pharyngeal muscle of the pharynx muscle was investigated by immunohistochemistry. After injection of PRV into the pharyngitis muscle, the appearance of PRV-labeled cells in the brain increased with the survival time of the animals. 48h after pharyngitis injection of PRV, positive labeling cells appeared only in the semi-dense part of suspected nucleus. After 56h of survival, the labeled cells could be found in the subnucleus, medial subnucleus and sub-nucleus in the nucleus of solitary tract. Survival of 62 ~ 72h, the medulla oblongata in the nuclear group, the paraloid, the lateral reticular nucleus, blue pons, parabrachial nuclei, A5 cells and other parts of a large number of labeled cells can be seen. Survival of 80 ~ 96h, the medulla oblongata and pons in the above parts of the marker cells are more dense, and in the trigeminal nucleus and Barington nuclear nuclei appear labeled cells. Central gray matter and midbrain deep nucleus in the midbrain; preoptic area, paraventricular nucleus, lateral zone, arcuate nucleus, anterior commissure nucleus in hypothalamus; paraventricular nucleus and lateral habenular nucleus of the thalamus; lateral septal nucleus of the telencephalon , The end of the bed bed nuclei, amygdala and other parts of a large number of positive cells can be seen. Animals that survived 102h had similar, but higher numbers of markers for their 96h survival. And in the cortical frontal area, medial and lateral central anterior precancerous cells appear more markers. Speculated that many parts of the brain and pharyngeal motoneurons neuronal regulation