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以怀牛膝悬浮细胞系为试材,对牛膝细胞悬浮培养动力学进行了研究,采用HPLC、原子吸收分光光度法和电导率测定等方法,分析了牛膝悬浮细胞生长、营养消耗和三萜皂苷积累的动力学关系。结果表明:牛膝细胞悬浮培养周期约为24d,经24d的悬浮培养,最大生物量和齐墩果酸含量分别达到了13.47mg/L和1.418mg/g;牛膝悬浮细胞生长与三萜皂苷积累的动力学关系属于生长偶联型。培养过程中培养液的pH值呈先下降后回升的趋势,而电导率逐渐减小,24d时降到最低点,而后略有上升。经15d培养,培养基中的蔗糖即消耗殆尽;培养21d后,培养基中大量的氮源、磷源、K+和Mg2+被消耗,而Ca2+比较充足,基本可以满足细胞生长的需要。由此可推断,培养液中营养成分的不足限制了细胞的快速增长和三萜皂苷含量的提高。
The suspension cell culture of Achyranthes bidentata was used as a test material to study the suspension culture kinetics of Achyranthes bidentata cells. The effects of suspension cell growth, nutrient consumption and three Kinetics of the accumulation of terpenoid saponins. The results showed that the cell cycle of Achyranthes bidentata cells was about 24 days. After 24 days of suspension culture, the maximum biomass and oleanolic acid content reached 13.47 mg / L and 1.418 mg / g, respectively. The growth of Achyranthes bidentata suspension cells was correlated with triterpene saponin The accumulated kinetic relationship belongs to the growth-coupled type. During culture, the pH value of the culture medium first decreased and then rose, while the conductivity decreased gradually and reached the lowest point on the 24th day, then slightly increased. After cultured for 15 days, the sucrose in the culture medium was depleted. After culturing for 21 days, a large amount of nitrogen and phosphorus sources, K + and Mg2 + were consumed in the culture medium, while Ca2 + was sufficient to basically meet the needs of cell growth. It can be inferred that the lack of nutrients in culture fluid limits the rapid growth of cells and triterpene saponin content.