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目的通过对汕头市病毒性腹泻的病原监测,了解其发病情况及病原组成。方法粪便标本由哨点医院采集,汕头市疾控中心用ELISA法检测轮状病毒抗原,用Real-time PCR检测诺如病毒、腺病毒及星状病毒的核酸。结果共检测719例腹泻病例标本,检出率从高到低依次为轮状病毒22.7%,诺如病毒11.3%,腺病毒1.0%,星状病毒0.6%,其中重叠感染1.3%;<16岁组病原检出率(44.4%)比≥16岁组(21.4%)高;<16岁组以轮状病毒为主(70.3%),≥16岁组以诺如病毒为主(51.7%);症状除100.0%腹泻外,发热和呕吐占50.0%左右;每年的8月至次年2月是高发期。结论轮状病毒和诺如病毒是汕头市病毒性腹泻的主要病原,应加强防控。
Objective To monitor the pathogen of viral diarrhea in Shantou City to understand its incidence and pathogenic composition. Methods Stool specimens were collected from sentinel hospital. Shantou CDC detected rotavirus antigen by ELISA, and real-time PCR was used to detect the DNA of norovirus, adenovirus and astrovirus. Results A total of 719 cases of diarrhea were detected. The detection rates were 22.7% for rotavirus, 11.3% for Norovirus, 1.0% for adenovirus and 0.6% for astrovirus, among which 1.3% were superinfection and <16 years The detection rate of the group of pathogens (44.4%) was higher than that of the group of ≥16 (21.4%); rotavirus was the most common in the group of 16 years old (70.3%); In addition to 100.0% of the symptoms of diarrhea, fever and vomiting accounted for 50.0%; annual August to February next year is the high incidence. Conclusions Rotavirus and Norovirus are the major pathogens of viral diarrhea in Shantou.