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目的：研究脑缺血中氧自由基（ＯＦＲ）和一氧化氮（ＮＯ）的动态变化及金丝桃甙（Ｈｙｐ）的作用。方法：脑缺血模型由结扎大鼠四血管造成。用低温电子顺磁共振（ＥＳＲ）技术和ｇｒｅｎ法分别测定脑皮质中的ＯＦＲ和ＮＯ。结果：脑缺血５ｍｉｎ时，ＮＯ就增多，１５ｍｉｎ时，ＯＦＲ才有增多；５０，１００ｍｇ·ｋｇ－１Ｈｙｐ显著减少，ＯＦＲ和ＮＯ的增高，抑制超氧化物歧化酶和乳酸脱氧酶活性的下降，延长小鼠断头后张口喘气时间。结论：脑缺血中，ＮＯ增多早于ＯＦＲ，Ｈｙｐ对脑缺血损伤有保护作用。
Objective: To study the dynamic changes of oxygen free radical (OFR) and nitric oxide (NO) during cerebral ischemia and the effect of hypericum perforatum (Hyp). METHODS: A model of cerebral ischemia was caused by ligation of four blood vessels in rats. The OFR and NO in cerebral cortex were measured by low-temperature electron paramagnetic resonance (ESR) technique and gren method. RESULTS: At 5 min after cerebral ischemia, NO increased. At 15 min, the OFR increased; 50, 100 mg·kg-1 Hyp decreased significantly, OFR and NO increased, and the activities of superoxide dismutase and lactate deoxygenase were inhibited. Extend the mouth breathing time after the mice become decapitated. Conclusion: In cerebral ischemia, the increase of NO is earlier than that of OFR. Hyp has protective effect on cerebral ischemic injury.