论文部分内容阅读
目的为实现对药用植物的可持续利用,对玉簪Hosta plantaginea的花进行了甾体皂苷类成分及其体外抗肿瘤作用的研究。方法应用常规柱色谱(包括正相、反相和凝胶柱色谱)分离和波谱分析方法对玉簪花进行分离和结构鉴定;以3种悬浮肿瘤细胞白血病肿瘤细胞株(HL-60、Jurkat、K562)和3种贴壁实体瘤细胞株(肝癌HepG2、乳腺癌MCF7、胃癌SGC7901)为研究对象,采用MTT法对化合物进行体外抗肿瘤活性筛选研究。结果从玉簪花中分离鉴定了10个化合物,其中3个化合物为首次从该植物中分离得到,分别为吉托皂苷元(1)、吉托皂苷元-3-O-β-D-葡萄糖(1→4)-β-D-半乳糖苷(3)、吉托皂苷元-3-O-{β-D-木糖(1→4)-β-D-葡萄糖(1→2)-[β-D-木糖(1→3)]-O-β-D-葡萄糖(1→4)-β-D-半乳糖苷}(10);7个已知化合物,分别是吉托皂苷元-3-O-β-D-半乳糖苷(2)、吉托皂苷元-3-O-α-L-鼠李糖(1→2)-β-D-半乳糖苷(4)、吉托皂苷元-3-O-β-D-葡萄糖(1→2)-β-D-葡萄糖(1→4)-β-D-半乳糖苷(5)、吉托皂苷元-3-O-β-D-葡萄糖(1→4)-O-[α-L-鼠李糖(1→2)]-β-D-半乳糖苷(6)、替告皂苷元-3-O-β-D-葡萄糖(1→4)-O-[α-L-鼠李糖(1→2)]-β-D-半乳糖苷(7)、吉托皂苷元-3-O-{β-D-葡萄糖(1→2)-O-[β-D-木糖(1→3)]-O-β-D-葡萄糖(1→4)-β-D-半乳糖苷}(8)、吉托皂苷元-3-O-β-D-葡萄糖(1→2)-O-[α-L-鼠李糖(1→4)-β-D-木糖(1→3)]-O-β-D-葡萄糖(1→4)-β-D-半乳糖苷(9)。体外抗肿瘤活性实验结果显示化合物5,6,8～10对肝癌HepG2、乳腺癌MCF7和胃癌SGC7901肿瘤细胞毒活性较强。结论玉簪花中的甾体化合物对不同肿瘤细胞具有细胞毒选择性,有一定的抗癌活性,同时利用地上部分的花作为药用资源,可以实现对该药用植物的可持续利用。
Aim To achieve the sustainable utilization of medicinal plants, the steroidal saponins of the flower of Hosta plantaginea and its antitumor activity in vitro were studied. Methods The constituents of Hosta were separated and identified by conventional column chromatography (including normal phase, reverse phase and gel column chromatography) and spectral analysis. Three kinds of tumor cell lines HL-60, Jurkat, K562 ) And three kinds of adherent solid tumor cell lines (HepG2, MCF7 and SGC7901) were used as the research objects. The anti-tumor activity of the compounds was evaluated by MTT assay. Results Ten compounds were isolated and identified from Hosta flowers. Three of the compounds were isolated from this plant for the first time, and were identified as GITAS (1), GIST-3-O-β-D-glucose 1 → 4) -β-D-glucose (1 → 4) -β-D-glucose (1 → 2) - [ β-D-xylose (1 → 3)] - O-β-D-glucose (1 → 4) -β-D-galactoside} (10); seven known compounds, -3-O-β-D-galactoside (2), krototenoid-3-O-α-L-rhamnose Tachykinin-3-O-β-D-glucose (1 → 2) -β-D-glucose (1 → 4) -β-D-galactoside β-D-glucose (1 → 4) -O- [α-L-rhamnose (1 → 2)] - β-D-galactoside D-glucose (1 → 4) -O- [α-L-rhamnose (1 → 2)] - β-D-galactoside Glucose (1 → 2) -O- [β-D-xylose (1 → 3)] - O-β-D-glucose (1 → 4) -β-D-galactoside} (1 → 2) -O- [α-L-rhamnose (1 → 4) -β-D-xylose (1 → 3)] - O- β-D-glucose (1 → 4) -β-D-galactoside (9). The antitumor activity in vitro showed that compounds 5, 6, 8 and 10 had strong cytotoxic activity against HepG2, MCF7 and SGC7901. Conclusion The steroidal compounds in Hosta flower have the cytotoxicity selectivity to different tumor cells and have certain anti-cancer activity. At the same time, using the aerial part of the flower as the medicinal resource, the sustainable use of the medicinal plant can be realized.