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目的 寻找可能存在的抗丙型肝炎病毒 (HCV)感染的中和抗体。方法 构建两个分别含HCVE1和E2抗原基因的真核表达载体 ,用瞬时表达法检测其在哺乳动物细胞中的表达。将构建的载体连同IL 2基因一起经皮下给BalB/c小鼠进行注射 ,然后用ELISA法检测特异性抗体产生情况。最后通过研究抗体和病毒间的相互作用分析基因免疫诱导产生的抗血清在体外与HCV的相互作用情况。结果 经过基因免疫的小鼠分别产生了E 1和E2抗体。其中 ,用含E2基因的质粒载体免疫的小鼠所产生的E2抗血清不仅可以免疫沉淀来源血清中的HCV病毒颗粒 ,而且可以和与来源株非相关的HCV病毒颗粒相互作用。结论 我们的研究表明基因免疫诱导小鼠产生的E2抗体可以和HCV病毒颗粒发生交叉反应 ,这使我们看到了诱导产生具有广泛针对性的E2抗体的希望。
Objective To search for possible neutralizing antibodies against hepatitis C virus (HCV) infection. Methods Two eukaryotic expression vectors containing HCVE1 and E2 antigen genes were constructed and their expression in mammalian cells was detected by transient expression. The constructed vector was injected subcutaneously into the BalB / c mice along with the IL2 gene, and then the specific antibody production was examined by ELISA. Finally, the interaction between antibody and virus was analyzed to analyze the interaction of antiserum induced by gene immunization with HCV in vitro. Results E 1 and E2 antibodies were generated in mice immunized respectively with the gene. Among them, the E2 antisera raised by the mice immunized with the plasmid vector containing the E2 gene not only immunoprecipitated the HCV virus particles in the source sera, but also interacted with the HCV virus particles unrelated to the source strain. Conclusions Our study shows that immunization of mice with E2 antibodies induced cross-reactivity with HCV virion allows us to see the hope of inducing the production of E2 antibodies with broad specificity.