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目的:应用RP-HPLC测定胃苏冲剂中柚皮甙含量。方法:用ShimpackCLC-ODS柱,甲醇醋酸水(35∶4∶61)为流动相,检测波长283nm。结果:柚皮甙量在0.12~1.02μg范围内与色谱峰面积呈线性关系;回归方程^Y=174140.32X+43.35,r=0.9997;平均加样回收率及RSD分别为98.7%和0.98%。结论:该法建立了胃苏冲剂质量控制标准。
Objective: To determine the content of naringin in Weisu granules by RP-HPLC. Methods: The mobile phase was ShimpackCLC-ODS column and methanol-acetic acid-water (35:4:61). The detection wavelength was 283nm. Results: The content of naringin in the range of 0.12 ~ 1.02μg was linear with the chromatographic peak area. The regression equation was Y = 174140.32X + 43.35, r = 0.9997. The average recovery and RSD were 98.7% and 0.98%. Conclusion: This method established the quality control standard of Weisu granules.