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目的研究赖氨匹林(aspisol)对人宫颈癌Hela细胞增殖、凋亡和周期的影响。方法取处于对数生长期的Hela细胞,实验分为4组:阴性对照组只加等体积的低糖DMEM培养液Hela细胞;实验组加入不同浓度的aspisol,使其终浓度分别为1 mmol/L、5 mmol/L、10 mmol/L。采用描计细胞生长曲线法检测细胞增殖状态;HE染色、AO/EB方法进行凋亡细胞形态学的观察。Annexin V-FITC/PI双染检测赖氨匹林对Hela细胞作用24 h后细胞凋亡情况;流式细胞术分析赖氨匹林对Hela细胞作用24 h后细胞周期的变化。结果与对照组比较,aspisol(1,5,10 mmol/L)可呈时间、浓度依赖性抑制Hela细胞的增殖;HE染色光镜下见Aspisol组细胞密度减小,细胞变圆,胞核染色变浅。赖氨匹林(1,5,10mmol/L)作用24 h后诱导Hela细胞的早期凋亡率分别为(5.73±0.87)%、(19.11±2.86)%、(33.72±5.06)%,与对照组(0.46±0.69)%比较,差异有统计学意义(P<0.01),并呈浓度依赖性。细胞周期检测显示赖氨匹林对Hela细胞有G0/G1期阻滞作用。结论 aspisol对宫颈癌Hela细胞有抑制增殖、诱导其凋亡和改变其细胞周期分布,阻滞Hela细胞于G0/G1期。
Objective To investigate the effects of aspisol on proliferation, apoptosis and cycle of human cervical carcinoma Hela cells. Methods Hela cells in logarithmic growth phase were collected and divided into four groups. The negative control group was given only HeLa cells with the same volume of low glucose DMEM culture medium. Different concentrations of aspisol were added to the experimental group to make the final concentration of 1 mmol / L , 5 mmol / L, 10 mmol / L. Cell proliferation was detected by scanning cell growth curve. Morphological changes of apoptotic cells were observed by HE staining and AO / EB. Annexin V-FITC / PI double staining was used to measure the apoptosis of Hela cells after 24 h. The changes of cell cycle of Hela cells treated with meticloprid were analyzed by flow cytometry. Results Compared with the control group, aspisol (1,5,10 mmol / L) could inhibit the proliferation of Hela cells in a time-and concentration-dependent manner. The cell density of Aspisol group decreased, the cells became round and the nuclei were stained shallow. The early apoptotic rates of Hela cells were (5.73 ± 0.87)%, (19.11 ± 2.86)% and (33.72 ± 5.06)%, respectively, after treated with 1,5, and 10 mmol / Group (0.46 ± 0.69)%, the difference was statistically significant (P <0.01), and in a concentration-dependent manner. Cell cycle assay showed that L-aminopyrine inhibited G0 / G1 arrest in Hela cells. Conclusion aspisol can inhibit the proliferation of cervical cancer Hela cells, induce apoptosis and change the cell cycle distribution, and arrest Hela cells in G0 / G1 phase.