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通过盆栽和大田试验，研究了籼型低温敏核不育系GD－2S的冷灌繁种技术。结果表明，1）冷灌开始时期以幼穗分化Ⅲ期（二次枝梗和颖花原基分化）为好。但是，GD－2S的温度诱导育性转换的敏感期在抽穗前的第11天至19天，即雌雄蕊形成期（Ⅳ期）至花粉母细胞减数分裂期（Ⅵ期）。冷灌处理开始至植株产生反应之间存在一个启动过程。2）冷灌天数以13d左右为宜。冷灌处理会导致颖花退化，延缓生育进程，推迟抽穗时间。冷灌开始时期越早或冷灌时间越长，颖花退化越严重（如冷灌20d的达25.5％），结实率也降低。3）冷灌深度以12cm左右（刚浸至生长点）深的结实率最高。
Through pot and field experiments, we studied the technology of cold irrigation and multiplication of indica genic male sterile line GD-2S. The results showed that 1) the stage of panicle differentiation (secondary branch and spike primordial differentiation) was better at the beginning of cold irrigation. However, the sensitivities of temperature-induced fertility transformation of GD-2S ranged from the 11th day to the 19th day before heading, ie, the stage of pistil and stamen (stage IV) to the meiosis of pollen mother cells (stage Ⅵ). There is a start-up process between the beginning of cold-filling and the reaction of plants. 2) cold irrigation days to 13d is appropriate. Cold irrigation will lead to degradation of spikes, delay the process of reproduction, postpone the heading time. The earlier the cold irrigation started or the longer the cold irrigation period, the more serious the spikelet degeneration (such as 25.5% of the 20 days of cold irrigation), the seed setting rate also decreased. 3) The depth of cold irrigation to about 12cm (just soaked to the growth point) the highest seed setting rate.