论文部分内容阅读
目的:建立葛连解糖颗粒的定性鉴别和含量测定方法。方法:采用薄层色谱法对葛连解糖颗粒中葛根、黄连、胡芦巴、枸杞子、地黄等进行定性分析;采用高效液相法,建立葛连解糖颗粒中葛根素、盐酸小檗碱的含量测定方法。结果:建立的薄层色谱鉴别方法,分别能检出葛根、黄连、枸杞子、地黄、胡芦巴药材,薄层斑点清晰,阴性对照均无干扰。葛根素进样量在21.1～337.6 ng(r=0.999 9),盐酸小檗碱进样量在43.1～689.9 ng范围内线性关系良好(r=0.999 9);葛根素平均回收率为97.21%,RSD为1.41%(n=6),盐酸小檗碱平均回收率为96.18%,RSD为1.22%(n=6)。结论:所建方法定性专属性强,定量准确度高,可用于葛连解糖颗粒的质量控制。
Objective: To establish a method for the qualitative identification and content determination of Ge Lian Jie Tang granules. Methods: The Puerarin, Coptidis, Coptis, Fructus Lycii and Rehmanniae Radix were analyzed qualitatively by thin-layer chromatography. Puerarin, Berberine hydrochloride Determination of alkali content. Results: The established TLC identification methods were able to detect Pueraria, Coptis, Lycium, Rehmannia, Fenugreek herbs, thin spots clear, negative control without interference. The injection volume of puerarin ranged from 21.1 to 337.6 ng (r = 0.999 9), and the linearity of berberine hydrochloride injection ranged from 43.1 to 689.9 ng (r = 0.999 9). The average recoveries of puerarin were 97.21% The RSD was 1.41% (n = 6). The average recovery of berberine hydrochloride was 96.18% with a RSD of 1.22% (n = 6). Conclusion: The established method is of high specificity and high specificity and can be used for the quality control of Ge Lian Jie Tang granules.