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目的观察脑损伤后碱性成纤维细胞生长因子(bFGF)及其受体FGFR1的表达及其时序性变化,探讨脑损伤的分子机制及法医学脑损伤时间推断。方法用免疫组织化学技术观察大鼠侧位中度液压冲击脑损伤后bFGF及FGFR1蛋白在伤后不同时间(30min,1,3,6,12h,1,3,7d)的表达情况,以正常大鼠及手术大鼠作为对照。结果正常对照组和手术对照组大鼠脑组织内有低水平的bFGF和FGFR1表达。脑冲击伤后6h,大脑皮层和脑干bFGF和FGFR1蛋白表达开始显著增加,1～3d达高峰,7d时已有所下降;海马冲击后3h即开始增加,1d达峰值,此后逐渐下降,7d时恢复正常。结论脑损伤可诱导bFGF/FGFR1的表达,提示机体对脑损伤存在自身保护机制,其表达的时序性改变可望用于法医学脑损伤时间推断。
Objective To observe the expression of bFGF and its receptor FGFR1 after brain injury, and to study the molecular mechanism of brain injury and forensic brain injury time. Methods The expression of bFGF and FGFR1 protein at different time points after injury (30 min, 1, 3, 6, 12 h, 1, 3 and 7 days) was observed by immunohistochemistry in normal rats. Rats and surgery rats served as controls. Results There were low levels of bFGF and FGFR1 expression in the brain tissue of normal control group and surgical control group. At 6h after brain injury, the expression of bFGF and FGFR1 protein in cerebral cortex and brainstem began to increase significantly, peaked at 1 ~ 3d and decreased at 7d. Hippocampal began to increase at 3h and peaked at 1d, and then decreased gradually. When returned to normal. Conclusions Brain damage can induce the expression of bFGF / FGFR1, suggesting that there is a self-protection mechanism of the body on brain injury. The temporal change of expression may be used to infer the time of brain injury in forensic medicine.