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目的了解慢性丙型肝炎患者和对照组血清ALT、AST、TBil、IL-17水平以及在丙型肝炎的不同发展阶段的变化特点,探讨病毒载量与IL-17、ALT、AST、TBil的相关性。方法 85例丙型肝炎患者其中包括24例丙型肝炎肝硬化患者和61例慢性丙型肝炎患者,30名健康人作为对照。所有丙型肝炎患者抗-HCV抗体检测S/CO>3.8(排除抗-HCV假阳性患者),研究对象分为4组。采用全自动生化分析仪检测所有研究对象的ALT、AST、TBil的水平,采用酶联免疫吸附实验-双抗体夹心法(ELISA)检测所有研究对象血清中抗-HCV抗体和细胞因子IL-17水平。采用荧光定量聚合酶链反应(RT-PCR)检测HCV-RNA的病毒载量。结果(1)通过各组间多重比较,丙型肝炎患者各组IL-17水平显著高于健康对照组(P<0.05),差异有统计学意义,在病毒载量>1×103组IL-17水平与ALT、AST水平变化有很好的相关性。(2)按照病毒载量HCV-RNA高低分组,病毒载量与IL-17水平呈正相关,随着病毒载量的升高IL-17水平也随之升高。结论患者组IL-17、ALT、AST、TBil的检测水平升高可能与患者肝细胞的炎性损伤有关,并且IL-17在一定程度上能够反映肝脏的损伤程度。在病毒检测阳性的患者中病毒载量与IL-17水平呈正相关。
Objective To investigate the changes of serum ALT, AST, TBil and IL-17 levels in patients with chronic hepatitis C and the control group at different stages of development of hepatitis C and to explore the correlation between viral load and IL-17, ALT, AST and TBil Sex. Methods Totally 85 patients with hepatitis C including 24 patients with hepatitis C cirrhosis and 61 patients with chronic hepatitis C and 30 healthy controls were enrolled in this study. All patients with hepatitis C were tested for anti-HCV antibody S / CO> 3.8 (excluding anti-HCV false-positive patients) and the subjects were divided into 4 groups. The levels of ALT, AST and TBil in all the subjects were detected by automatic biochemical analyzer. The levels of anti-HCV antibody and cytokine IL-17 in sera of all subjects were detected by enzyme-linked immunosorbent assay (ELISA) . The viral load of HCV-RNA was detected by fluorescence quantitative polymerase chain reaction (RT-PCR). Results (1) The levels of IL-17 in each group of patients with hepatitis C were significantly higher than those in healthy controls (P <0.05) by multiple comparisons among groups. The difference was statistically significant. In IL- 17 levels and ALT, AST level changes have a good correlation. (2) According to the level of HCV-RNA, the viral load was positively correlated with the level of IL-17. With the increase of viral load, IL-17 level also increased. Conclusion The elevated levels of IL-17, ALT, AST and TBil in patients may be related to the inflammatory injury of hepatocytes in patients. IL-17, to a certain extent, can reflect the degree of liver damage. There was a positive correlation between viral load and IL-17 levels in patients tested positive for virus.