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以菊花‘雨花落英’ב奥运含笑’F1群体为材料,调查了2008—2009两个年度开花持续期的分离表现,并基于菊花SRAP遗传图对其进行QTL定位分析。基于单年数据,运用Win QTL Cartographerv2.5软件及复合区间作图法在2008年共检测到3个控制开花持续期的加性QTL,主要分布在秋菊‘雨花落英’遗传图的Y20和Y42连锁群以及夏菊‘奥运含笑’遗传图的A14连锁群上,LOD值介于2.65~3.44之间,单个QTL对开花持续期表型变异的贡献率为6.54%~11.58%,而在2009年未检测到QTL的存在。运用QTL Networkv2.2软件对2008和2009两年的表型数据进行联合分析,共检测到两对上位性QTL,在贡献率和效应值上与加性QTL相当,同时上位性QTL与环境之间具有互作效应。
Chrysanthemum ’Yuhualuoxing’ × ’Olympic smile’ F1 population was used as material to investigate the segregation of flowering duration in 2008-2009 and to conduct QTL mapping based on the chrysanthemum SRAP genetic map. Based on the single-year data, three QTLs controlling flowering durations were detected using Win QTL Cartographerv2.5 software and composite interval mapping method in 2008, mainly distributed in Y20 Y42 linkage group and Xiaju ’Olympic Smiley’ genetic linkage map, the LOD values ranged from 2.65 to 3.44. The single QTL contributed 6.54% ~ 11.58% to the phenotypic variation of flowering duration, while in 2009 No QTLs were detected in the year. Using QTL Networkv2.2 software to analyze the phenotypic data of 2008 and 2009, we detected two pairs of epistatic QTLs, which were similar to additive QTLs in contribution rate and effect value. Meanwhile, between epistatic QTLs and environment With the interaction effect.