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目的:观察内脂素(visfatin)诱导人类单核细胞白血病细胞株THP-1源性泡沫细胞形成中过氧化物酶体增殖物激活受体γ(PPARγ)的表达作用。方法:给予不同浓度的visfatin处理THP-1源性巨噬细胞,并加入低密度脂蛋白(LDL)孵育24 h后,运用油红O染色观察细胞内脂滴的变化,运用逆转录聚合酶链反应和Western-blot检测PPARγ的mRNA和蛋白表达水平。结果:高浓度的visfatin刺激负荷LDL的THP-1源性巨噬细胞24 h后,细胞质内的脂滴明显增多。visfatin呈浓度依赖性的下调负荷LDL的THP-1源性巨噬细胞中PPARγmRNA和蛋白表达(P<0.05)。结论:visfatin对THP-1源性泡沫细胞形成中PPARγ表达水平的下调,这可能在动脉粥样硬化的发生发展起重要作用。
Objective: To observe the expression of peroxisome proliferator - activated receptor γ (PPARγ) in visfatin - induced human monocytic leukemia cell line THP-1 derived foam cells. Methods: THP-1-derived macrophages were treated with different concentrations of visfatin and incubated with low density lipoprotein (LDL) for 24 h. The changes of intracellular lipid droplets were observed by oil red O staining. The reverse transcription polymerase chain The mRNA and protein expression levels of PPARγ were detected by Western blot and Western blot. Results: High concentrations of visfatin stimulated THP-1-derived macrophages loaded with LDL for 24 h, and lipid droplets in the cytoplasm increased significantly. Visfatin down-regulated the mRNA and protein expression of PPARγ in THP-1-derived macrophages loaded with LDL in a concentration-dependent manner (P <0.05). CONCLUSION: Visfatin down-regulates the expression of PPARγ in THP-1-derived foam cells, which may play an important role in the development of atherosclerosis.