论文部分内容阅读
GM-CSF和IL-3是两种功能相关,作用于造血系统的糖蛋白。它们对红系、髓系和造血祖细胞均有刺激作用。GM-CSF和IL-3的受体存在于多种造血细胞表面。为进一步研究GM-CSF和IL-3的协同作用,作者构建了一种新的融合蛋白GM-CSF-IL-3(PlXY 321),此蛋白在受体结合,促细胞增殖,促造血细胞集落形成诸方面的功能均高子GM-CSF、IL-3单独使用时的功能。作者通过编吗(Gly-Gly-Gly-Gly-Ser)的寡核苷酸接头将GM-CSF和IL-3两者的cDNA连接起来,并插入酵母表达载体中表达,表达产物经SDS-PAGE电泳测定其分子量为35kD,与预期分子量一
GM-CSF and IL-3 are two functionally related glycoproteins that act on the hematopoietic system. They stimulate erythroid, myeloid and hematopoietic progenitor cells. Receptors for GM-CSF and IL-3 are found on the surface of many hematopoietic cells. To further investigate the synergistic effect of GM-CSF and IL-3, the authors constructed a novel fusion protein GM-CSF-IL-3 (PlXY321) that binds receptors, promotes cell proliferation, Formed in all aspects of the function of GM-CSF, IL-3 alone when the function. The authors linked cDNA of both GM-CSF and IL-3 by ligating the cDNA of Gly-Gly-Gly-Gly-Ser and inserted into the yeast expression vector for expression. The expressed product was analyzed by SDS-PAGE The molecular weight of 35kD was determined by electrophoresis and the expected molecular weight was 1