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目的:探讨血管内皮生长因子(VEGF)及胰岛素样生长因子-1(IGF-1)在窒息新生儿脐血水平变化的临床意义。方法:采用双抗体夹心酶联免疫吸附法(ELISA)及放射免疫分析法(RIA)测定正常新生儿(67例,对照组)和窒息新生儿(50例,窒息组)脐血血管内皮生长因子(VEGF)及胰岛素样生长因子-1(IGF-1)的水平。结果:新生儿窒息时脐血VEGF比正常脐血对照组显著升高(t=-9.944,p<0.01),IGF-1比正常脐血对照组显著下降(t=-15.943,p<0.01)。结论:窒息新生儿VEGF水平的上升与IGF-1水平的下降,提示两者均可能参与新生儿窒息的病理生理过程,同时检测新生儿脐血VEGF与IGF-1可望成为反映新生儿窒息程度的一个敏感和特异的指标。
Objective: To investigate the clinical significance of vascular endothelial growth factor (VEGF) and insulin-like growth factor-1 (IGF-1) in umbilical cord blood of asphyxial neonates. Methods: The levels of umbilical vascular endothelial growth factor (VEGF) in normal neonates (67 cases, control group) and asphyxial neonates (50 cases, asphyxia group) were determined by ELISA and radioimmunoassay (RIA) (VEGF) and insulin-like growth factor-1 (IGF-1) levels. Results: The expression of VEGF in neonatal asphyxia increased significantly (t = -9.944, p <0.01), and decreased significantly in normal umbilical cord blood (t = -15.943, p <0.01) . Conclusion: Asphyxia neonatal VEGF levels and IGF-1 levels decline, suggesting that both may be involved in the pathophysiology of neonatal asphyxia, while detection of neonatal cord blood VEGF and IGF-1 is expected to be a reflection of neonatal asphyxia A sensitive and specific indicator.