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软骨细胞和骨细胞都有很多的细胞突起,骨细胞并以长突互相交联形成细胞网络。由于骨细胞突起穿行于骨基质内,用通常的方法处理标本进行扫描电镜观察是极其困难的。本文利用盐酸—蛋白酶水解消化法,去除软骨及骨基质,暴露非游离缘的细胞,观察二次骨化中心到原始骨小梁各层细胞的三维结构。生后四周大鼠,乙醚麻醉,3％二甲砷酸钠缓冲的戊二醛溶液经左心室灌流,取出胫骨,剔除周围软组织,冰冻裂断后,投入PBS配制的2.5 mg/m的胰蛋白酶溶液,PH7.437C消化30分钟,照出后立即投入8NHCL内60℃振荡。清洗后,梯度乙醇脱水,醋酸异戊脂浸透,临界点干燥,真空喷镀,日立S—500A型扫
Chondrocytes and bone cells have a lot of cell protrusions, osteocytes and long protrusions cross each other to form a network of cells. Due to the osteoblastic processes running through the bone matrix, it is extremely difficult to treat specimens by scanning electron microscopy in the usual way. In this paper, hydrochloric acid-protease hydrolysis digestion, removal of cartilage and bone matrix, exposure to non-free edge cells, the secondary ossification center to observe the three-dimensional structure of the original trabecular layer cells. Rats were anesthetized with ether and anesthetized with ether 3%, and glutaraldehyde 3% cacodylide solution was perfused through the left ventricle. The tibia was removed and the surrounding soft tissue was removed. After freezing and thawing, the solution was put into a 2.5 mg / ml trypsin solution , PH7.437C digestion 30 minutes, according to immediately put into 8NHCL 60 ℃ oscillation. After cleaning, the gradient of ethanol dehydration, soaked in isopentyl acetate, the critical point of dry, vacuum plating, Hitachi S-500A-type scan