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目的 :建立适合国人尿液中头孢克洛分离分析的毛细管电泳方法。方法 :用重蒸馏水多倍稀释尿液后直接进样电泳分析。电泳分离条件为 :未涂层毛细管 48cm× 5 0μm ;运行缓冲液为 5 0 m mol/ L磷酸二氢钠 - 12 .5 mm ol/ L硼砂 (p H 8.0 8) ;操作电压 2 0 k V(+ )→ (- ) ;压力进样 8.2 74× 10 4Pa· s;柱上紫外 2 6 5 nm检测。 结果 :头孢克洛和内标物头孢拉定在 6 .5min内获得了理想的分离。尿药浓度在 2 .5～ 2 5 0 .0μg/ ml范围内线性关系良好 (r =0 .9991,n =5 ) ,绝对回收率大于95 .5 % ,日内及日间精密度均小于 8.0 %。 结论 :毛细管电泳应用于健康志愿者口服头孢克洛普通胶囊后尿药浓度的动态分析 ,方法准确可靠 ,操作简便易行
Objective: To establish a capillary electrophoresis method suitable for the separation and analysis of cefaclor in Chinese urine. Methods: Dilute urine with multiple distilled water and inject directly into the sample. Electrophoretic separation conditions were: uncoated capillary 48 cm × 50 μm; running buffer 50 μmol / L sodium dihydrogen phosphate - 12.5 mm ol / L borax (p H 8.08); operating voltage 20 k V (+) → (-); pressure injection 8.2 74 × 10 4 Pa · s; UV detection at 265 nm. RESULTS: Cefaclor and the internal standard cephradine achieved the ideal separation in 6.5 minutes. Urinary drug concentration in the range of 2.5 ~ 250.0μg / ml good linear relationship (r = 0.9991, n = 5), the absolute recovery was greater than 95.5%, intra-day and intra-day precision were less than 8.0 %. Conclusion: The capillary electrophoresis is applied to the dynamic analysis of urine concentration of cefaclor capsules in healthy volunteers. The method is accurate, reliable and easy to operate