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本研究以星星草(Puccinellia tenuiflora)种子在愈伤组织诱导培养基上萌发长出的纤细幼苗为亲本对照,分别应用AFLP和S-SAP技术,对其诱导的愈伤组织及其体外再生植株等体细胞无性系的遗传稳定性进行了分析。结果表明,以星星草亲本对照和随机选取的同起源的3瓶愈伤组织、8株星星草再生苗基因组DNA为模板,分别应用19对AFLP引物进行选择性扩增反应,结果表明,在电泳图谱上共检测到925条DNA条带,其中16条DNA片段呈多态性,遗传变异频率为1.7%;同时,在上述分析样品中,应用24对S-SAP选择性扩增引物共扩增出的789条DNA片段,其中36个位点具有多态性,变异频率达到了4.6%。聚类分析结果表明基于AFLP和S-SAP数据分析的样本彼此间平均遗传相似性系数分别为0.995和为0.987。
In this study, the seedlings of Puccinellia tenuiflora seeds, which grew on the callus induction medium, were used as the parental control. AFLP and S-SAP techniques were used respectively to induce callus and its regenerated plants in vitro The genetic stability of somatic clones was analyzed. The results showed that using 19 pairs of AFLP primers for selective amplification of the genomic DNA of three species of callus of the same origin and 3 randomly selected callus of the genus Asclepias, A total of 925 DNA bands were detected on the map, of which 16 were polymorphic and the genetic variation frequency was 1.7%. Meanwhile, 24 pairs of S-SAP selective amplification primers were used for co-amplification in the above analysis samples Out of 789 DNA fragments, of which 36 loci polymorphism, mutation frequency reached 4.6%. Cluster analysis showed that the average genetic similarity coefficients of the samples based on AFLP and S-SAP data analysis were 0.995 and 0.987, respectively.